Optimized platelet rich plasma releasate (O-rPRP) repairs galactosemia-induced ovarian follicular loss in rats by activating mTOR signaling and inhibiting apoptosis
| Autor: | Wesam El Bakly, Marwa Medhat, Yousef Shoukry, Mohamed Shafei, Nesreen Nabil Omar, Mohamed Fathi Mohamed Elrefai, Reham Fathy Tash |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Alternative medicine Cell biology Normal diet Ovarian insufficiency Apoptosis Premature ovarian insufficiency Toxicology 03 medical and health sciences Follicle-stimulating hormone 0302 clinical medicine Internal medicine Follicular phase medicine lcsh:Social sciences (General) lcsh:Science (General) PI3K/AKT/mTOR pathway Multidisciplinary Reproductive system Chemistry Galactosemia medicine.disease 030104 developmental biology Endocrinology Platelet-rich plasma Regenerative medicine mTOR lcsh:H1-99 Platelet rich plasma 030217 neurology & neurosurgery Reproductive hormone Hormone lcsh:Q1-390 Research Article |
| Zdroj: | Heliyon Heliyon, Vol 6, Iss 9, Pp e05006-(2020) |
| ISSN: | 2405-8440 |
| Popis: | Platelet rich plasma contains a collection of growth factors, and an optimal formulation, named O-rPRP, contains the highest possible concentration of growth factors. Purpose Challenging the healing power of O-rPRP in a high-galactose diet-induced premature ovarian insufficiency (POI) experimental rat model. Methods Rats were divided into four groups of ten rats each and treated for four week as follows; 1) the control group, fed with normal diet and received intraperitoneal (i.p.) injection of PBS once/week; 2) the POI group, fed with galactose diet (50%) and received PBS (i.p.) once/week; 3) the POI/O-rPRP group, fed a 50% galactose diet and received O-rPRP (i.p.) once/week; 4) the O-rPRP group (negative control), fed with a normal diet and received O-rPRP (i.p.) once/week. The levels of galactose, follicle stimulating hormone, 17 β-estradiol, anti-mullerian hormone and inhibin B were measured in serum samples. Western blotting and quantitative real-time PCR assays were employed to investigate the levels of miR-223, β1 integrin, p70S6k and MCL-1 in ovarian tissues. Results After O-rPRP treatment, β1 integrin expression was enhanced, and miR-223 expression was decreased. Unlike the untreated galactose group, in the group treated with O-rPRP, p70S6k and MCL-1 expression levels were increased, indicating that the mTOR growth signaling pathway was active and that apoptosis was inactive. After the introduction of O-rPRP, the number of follicles and the follicular maturation improved, which was consistent with the improvement of inhibin B levels and subsequent inhibition of FSH. Conclusion O-rPRP inhibited galactose-induced excessive atresia and provided an overall protective effect on the ovarian follicles. Alternative medicine; Regenerative medicine; Reproductive hormone; Reproductive system; Toxicology; Cell biology; Apoptosis; Galactosemia; mTOR; Ovarian insufficiency; Platelet rich plasma. |
| Databáze: | OpenAIRE |
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