Noninvasive analysis of skin proteins in healthy Chinese subjects using an Orbitrap Fusion Tribrid mass spectrometer
| Autor: | Sen Yang, Jinping Gao, Xuejun Zhang, Jing Zhang, Qi Zhen, Danfeng Qian, Yaochi Wang, Xiaomeng Wang, Mengting Liu, Huimin Guo, Xueying Li, Jie Ma, Ling Jin, Mingshun Wu, Shuangjun Xu |
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| Rok vydání: | 2018 |
| Předmět: |
Proteomics
Skin barrier Dermatology Computational biology Filaggrin Proteins Mass spectrometry Orbitrap 01 natural sciences Mass Spectrometry law.invention 010309 optics Cornified envelope 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine Asian People Intermediate Filament Proteins law 0103 physical sciences Keratin Protein purification Medicine Chinese subjects Humans Skin chemistry.chemical_classification integumentary system business.industry Proteins Healthy Volunteers chemistry Proteome Epidermis business Software |
| Zdroj: | Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI). 25(4) |
| ISSN: | 1600-0846 |
| Popis: | Objective The objective of this study was to perform noninvasive analysis of skin proteins in a healthy Chinese population using label-free nanoflow liquid chromatography-mass spectrometry (nLC-MS). Materials and methods Five consecutive tape strippings were obtained from the volar forearm skin of healthy Chinese subjects. Proteins were extracted, and trypsin-digested peptides were analyzed by a nanochromatography instrument coupled to an Orbitrap Fusion Tribrid mass spectrometer. Data-dependent acquisition allowed protein identification, which was performed by using Proteome Discoverer software (v2.2). Results In this study, we identified 80 common proteins that were expressed in the skin of healthy Chinese volunteers and divided these proteins into 16 categories, including keratins, cornified envelope proteins, and enzymes associated with substance metabolism. These proteins were closely associated with multiple functions of the skin barrier. Conclusion This study provides a noninvasive method to analyze healthy human epidermal proteins, which are closely associated with the skin barrier. In addition, this study provides a reference for further studies on the application of proteomic technologies to investigate the role of human epidermal proteins in health and disease. |
| Databáze: | OpenAIRE |
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