Murine brain tumor microenvironment immunophenotyping using mass cytometry
| Autor: | Brandon L. McClellan, Mahmoud S. Alghamri, Rohit Thalla, Pedro R. Lowenstein, Maria G. Castro |
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| Rok vydání: | 2022 |
| Předmět: |
General Immunology and Microbiology
Brain Neoplasms General Neuroscience FOS: Clinical medicine Immunology Single Cell Brain Cell Biology Glioma General Biochemistry Genetics and Molecular Biology Immunophenotyping Disease Models Animal Mice Model Organisms DNA Transposable Elements Tumor Microenvironment Animals Flow Cytometry/Mass Cytometry Antibody Cancer Neuroscience |
| Zdroj: | STAR protocols. 3(2) |
| ISSN: | 2666-1667 |
| Popis: | Here, we present a mass cytometry protocol optimized to examine the phenotype of immune cells within the mouse glioma microenvironment, using a Sleeping Beauty transposon-mediated mouse glioma model. We describe antibody conjugation and titrations for analysis of immune cells. We then detail mouse brain tumor tissue collection and processing, staining, followed by data acquisition, analysis, and gating strategy. This protocol can be applied to any brain tumor-harboring mouse model. For complete details on the use and execution of this protocol, please refer to Alghamri et al. (2021). |
| Databáze: | OpenAIRE |
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