| Autor: |
Motohiro Kato, Susumu Goyama, Nobutaka Kiyokawa, Kenichiro Hata, Kimikazu Matsumoto, Toshio Kitamura, Seishi Ogawa, Takeshi Inukai, Junko Takita, Kentaro Ohki, Masafumi Seki, Ryota Shirai, Hitomi Ueno-Yokohata, Hiroe Kizawa, Toru Uchiyama, Hiroko Ogata-Kawata, Masahiro Migita, Kazuko Hamamoto, Shohei Yamamoto, Tsukasa Hori, Hiroyuki Takahashi, Akihiro Watanabe, Daisuke Tomizawa, Masanori Yoshida, Kohji Okamura, Kazuhiko Nakabayashi, Meri Uchiyama, Moe Tamura, Shin-ichi Tsujimoto, Tomoo Osumi |
| Rok vydání: |
2023 |
| Popis: |
Supplementary Methods: IRB approval, Genomic analysis, Identification of translocations and determination of their breakpoints, Plasmids Construct, Cell line and reagent, Co-immunoprecipotation and immunoblotting analysis, Luciferase assay, Retrovirus production and transduction, FCM and Morphologic analysis, Human CB cell culture, and Colony replating assay. Supplementary Tables: Table 1. Clinical characteristics of RARA translocation negative APL cases. Table 2. BAC clones for FISH used in this study. Table 3. Primer sequences. Table 4. Somatic mutations observed in RARA-negative APL cases. Supplementary Figures: Figure 1. Morphologic feature and FISH analyisis for RARB translocations. Figure 2. Event-free survival of RARA-negative APL. Figure 3. Determination of TBL1XR1 mutated allele by allele-specific PCR. Figure 4. Constructs of TBL1XR1-RARB and other RARA fusions. Figure 5. Morphologic change in U937. Figure 6. Colony replating assay with mouse bone marrow. Figure 7. Colony replating assay with human cord blood. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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