Eph receptors and their ephrin ligands are expressed in developing mouse pancreas
| Autor: | Christophe E. Pierreux, Frédéric P. Lemaigre, Guy G. Rousseau, Lara Passante, Pierre Vanderhaeghen, Jonathan van Eyll |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
medicine.medical_specialty
Population Morphogenesis Biology Ligands EPH receptor B2 Mice Internal medicine Genetics medicine Ephrin Animals Receptor education Molecular Biology Pancreas In Situ Hybridization Receptors Eph Family education.field_of_study Reverse Transcriptase Polymerase Chain Reaction Erythropoietin-producing hepatocellular (Eph) receptor Gene Expression Regulation Developmental Immunohistochemistry biological factors Epithelium Cell biology medicine.anatomical_structure Endocrinology biological phenomena cell phenomena and immunity Ephrins Developmental Biology |
| Zdroj: | Gene expression patterns : GEP. 6(4) |
| ISSN: | 1567-133X |
| Popis: | Pancreas development involves branching morphogenesis concomitantly to differentiation of endocrine, exocrine and ductal cell types from a single population of pancreatic precursors. These processes depend on many signals and factors that also control development of the central nervous system. In the latter, Eph receptors and their class-A (GPI-anchored) and class-B (transmembrane) ephrin ligands control cell migration and axon-pathfinding, help establish regional patterns and act as labels for cell positioning. This raised the question as to whether and where Ephs and ephrins are expressed during pancreas development. Here we have identified the Eph and ephrin genes that are expressed in mouse embryonic pancreas, as detected by RT-PCR analysis. In situ hybridization experiments showed that Ephs and ephrins are mainly expressed in the burgeoning structures of the epithelium which differentiate into exocrine acini. Binding experiments on whole pancreas demonstrated the presence of functional Eph receptors. They showed that EphBs are expressed by the pancreatic epithelium at embryonic day (e) 12.5 and that, from e14.5 on, Ephs of both classes are expressed by the pancreatic epithelium and then become restricted to developing acini. We conclude that specific members of the Eph/ephrin family are expressed in embryonic pancreas according to a dynamic temporal and regional pattern. |
| Databáze: | OpenAIRE |
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