Comparison of Piezo-Assisted Micromanipulation with Conventional Micromanipulation for Intracytoplasmic Sperm Injection into Human Oocytes
| Autor: | Xiaoxiong Shen, Hiroyuki Minoura, Noritaka Futamura, Shigeto Takeuchi, Takashi Shibahara, Nagayasu Toyoda |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Male medicine.medical_treatment Semen Biology Intracytoplasmic sperm injection Andrology Human fertilization Pregnancy medicine Humans In patient Sperm Injections Intracytoplasmic Zona pellucida reproductive and urinary physiology Retrospective Studies In vitro fertilisation urogenital system Pregnancy Outcome Pipette Obstetrics and Gynecology Oocyte Spermatozoa medicine.anatomical_structure Reproductive Medicine embryonic structures Oocytes Female therapeutics |
| Zdroj: | Gynecologic and Obstetric Investigation. 52:158-162 |
| ISSN: | 1423-002X 0378-7346 |
| DOI: | 10.1159/000052965 |
| Popis: | Objective: To compare the efficacy of piezo-assisted micromanipulation with conventional micromanipulation for intracytoplasmic sperm injection (ICSI) into oocytes in patients with impaired semen parameters and no success with in vitro fertilization (IVF). Study Design: A retrospective randomized study was conducted on 204 cycles for 104 couples with piezo-assisted ICSI and 122 cycles for 96 couples with conventional ICSI. Piezo-assisted ICSI consists of two steps, namely penetration of the zona pellucida alone with a piezo-pulse and then puncturing of the oolemma with a light negative pressure without piezo, as with conventional ICSI. The tips of injection pipettes were prepared after pulling by breakage with a scalpel under a microscope, so that the inner diameter at and near the tip was 5 µm, as for conventional ICSI. Results: Piezo-assisted ICSI demonstrated significantly more favorable results, with a fertilization rate of 90.3% (conventional ICSI: 83.1%, p < 0.01) and a cleavage rate of 88.1% (conventional ICSI: 84.6%, p < 0.01). Conclusion: Piezo-assisted ICSI is easy to incorporate a spermatozoa exactly into the ooplasm with little deformation of the oocyte during insertion. Piezo-assisted ICSI can be used effectively for human oocytes to improve the fertilization, cleavage rates. |
| Databáze: | OpenAIRE |
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