The presence of Helicobacter pylori in the liver depends on the Th1, Th17 and Treg cytokine profile of the patient
| Autor: | Fabrício Freire de Melo, Gifone A. Rocha, Guilherme Santiago Mendes, Teresa C. A. Ferrari, Márcia Maria Negreiros Pinto Rocha, Dulciene M.M. Queiroz, Juliana B. Guerra, Agnaldo Soares Lima, Andreia Maria Camargos Rocha, Renato Dani, Lúcia Porto Fonseca de Castro, Sílvia B. Moura, Luciana Diniz Silva |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Adult
DNA Bacterial Male Microbiology (medical) Alcoholic liver disease lcsh:Arctic medicine. Tropical medicine Adolescent lcsh:RC955-962 liver diseases Hepatitis C virus medicine.medical_treatment lcsh:QR1-502 Enzyme-Linked Immunosorbent Assay Autoimmune hepatitis medicine.disease_cause DNA Ribosomal Polymerase Chain Reaction lcsh:Microbiology Helicobacter Infections Young Adult medicine Humans Prospective Studies Helicobacter IFN-γ Aged Aged 80 and over Hepatitis B virus biology Helicobacter pylori Liver Diseases Middle Aged Th1 Cells biology.organism_classification medicine.disease Immunohistochemistry Interleukin 10 IL-17 Cytokine Liver Case-Control Studies Immunology IL-10 Cytokines Th17 Cells Female |
| Zdroj: | Memórias do Instituto Oswaldo Cruz, Volume: 106, Issue: 6, Pages: 748-754, Published: SEP 2011 Memórias do Instituto Oswaldo Cruz., Vol 106, Iss 6, Pp 748-754 (2011) |
| Popis: | The hypothesis that Helicobactermight be a risk factor for human liver diseases has arisen after the detection of Helicobacter DNA in hepatic tissue of patients with hepatobiliary diseases. Nevertheless, no explanation that justifies the presence of the bacterium in the human liver has been proposed. We evaluated the presence of Helicobacterin the liver of patients with hepatic diseases of different aetiologies. We prospectively evaluated 147 patients (106 with primary hepatic diseases and 41 with hepatic metastatic tumours) and 20 liver donors as controls. Helicobacter species were investigated in the liver by culture and specific 16S rDNA nested-polymerase chain reaction followed by sequencing. Serum and hepatic levels of representative cytokines of T regulatory cell, T helper (Th)1 and Th17 cell lineages were determined using enzyme linked immunosorbent assay. The data were evaluated using logistic models. Detection of Helicobacter pylori DNA in the liver was independently associated with hepatitis B virus/hepatitis C virus, pancreatic carcinoma and a cytokine pattern characterised by high interleukin (IL)-10, low/absent interferon-γ and decreased IL-17A concentrations (p < 10-3). The bacterial DNA was never detected in the liver of patients with alcoholic cirrhosis and autoimmune hepatitis that are associated with Th1/Th17 polarisation. H. pylori may be observed in the liver of patients with certain hepatic and pancreatic diseases, but this might depend on the patient cytokine profile. |
| Databáze: | OpenAIRE |
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