Laboratory validation of a lateral flow device for the detection of CyHV-3 antigens in gill swabs
| Autor: | Maygane Ronsmans, Nesya Goris, Alain Vanderplasschen, Charles Mélard, T. Leclipteux, Maxime Boutier, Robert Vrancken, Johan Neyts, Alfred Collard, Anca Reschner, Stefaan Wera, François Lieffrig |
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| Rok vydání: | 2013 |
| Předmět: |
Gills
Veterinary Medicine Viral isolate Carps medicine.drug_class Cyprinid herpesvirus 3 ved/biology.organism_classification_rank.species Antibodies Viral Monoclonal antibody Sensitivity and Specificity Virus Fish Diseases Antigen Virology medicine Animals Koi herpesvirus Carp Antigens Viral Herpesviridae Plaque-forming unit Immunoassay biology ved/biology Antibodies Monoclonal Herpesviridae Infections biology.organism_classification |
| Zdroj: | Journal of Virological Methods. 193:679-682 |
| ISSN: | 0166-0934 |
| DOI: | 10.1016/j.jviromet.2013.07.034 |
| Popis: | a b s t r a c t Cyprinid herpesvirus-3 (CyHV-3) induces the highly contagious koi herpesvirus disease (KHVD) and may result in significant economic losses to the ornamental and food-producing carp industry. Suspicion of KHVD is triggered by clinical signs and confirmed using laboratory techniques. The latter are labour- and time-consuming, require specialised equipment and trained personnel. For rapid, on-site detection of CyHV-3, a lateral flow device (LFD) was developed using two monoclonal antibodies directed towards the viral glycoprotein ORF65. The LFD was highly specific with analytical and diagnostic specificities of 100%. Analytical sensitivity ranged between 1.25 × 102 and 2.40 × 104 plaque forming units per ml for isolates originating from geographically distinct regions. In experimentally infected carp, CyHV-3 was detected as early as 4-5 days post infection. Diagnostic sensitivities of 52.6% and 72.2% relative to PCR were recorded, depending on the viral isolate used. When onset of mortality was taken as reference, diagnostic sensitivities increased to 67.0% and 93.3%. The diagnostic sensitivity for freshly found-dead animals was 100%, irrespective of the virus isolate used. Given the high specificity and ease-of-use for on-site detection of CyHV-3, the LFD was regarded fit for purpose as a first-line diagnostic tool for the identification of acute CyHV-3 infections in KHVD affected (koi) carp. |
| Databáze: | OpenAIRE |
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