n-3 PUFA attenuate lipopolysaccharide-induced down-regulation of toll-like receptor 4 expression in porcine adipose tissue but does not alter the expression of other immune modulators
| Autor: | Doug M. Webel, Nicholas K. Gabler, Joel D. Spencer, Michael E. Spurlock |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Lipopolysaccharides
Male medicine.medical_specialty Fever Swine Endocrinology Diabetes and Metabolism Adipose tissue macrophages Clinical Biochemistry Adipose tissue Down-Regulation Gene Expression Inflammation Biology AMP-Activated Protein Kinases Protein Serine-Threonine Kinases Arachidonate 12-Lipoxygenase Biochemistry Multienzyme Complexes Internal medicine Fatty Acids Omega-3 medicine Animals RNA Messenger Muscle Skeletal Molecular Biology Unsaturated fatty acid Nutrition and Dietetics Tumor Necrosis Factor-alpha Eicosapentaenoic acid Diet Toll-Like Receptor 4 Endocrinology Adipose Tissue Docosahexaenoic acid Cyclooxygenase 2 TLR4 lipids (amino acids peptides and proteins) Tumor necrosis factor alpha medicine.symptom Orchiectomy |
| Zdroj: | The Journal of nutritional biochemistry. 19(1) |
| ISSN: | 0955-2863 |
| Popis: | The objective of this study was to test the hypothesis that the inflammatory response to lipopolysaccharide (LPS) in vivo is accompanied by down-regulation of toll-like receptor (TLR) 4 in adipose tissue, and a source of protected n-3 polyunsaturated fatty acid (PUFA) attenuates this response. Seventy-two castrated male pigs were individually fed either a control (CONT) diet, or the CONT diet containing 1.87% (LF) or 7.50% (HF) protected n-3 PUFA on a weight basis for 7 weeks. Adipose and muscle tissue biopsy samples were taken at Weeks 1, 2, 3, 4 and 7 to assess gene expression and/or confirm tissue enrichment with eicosapentaenoic acid and docosahexaenoic acid and reflected the n-3 PUFA contained in the diet. The LPS challenge was performed at week 7 and consisted of sequential injections of 10 and 2.5 mug LPS per kilogram of body weight 23 h apart. The LPS challenge resulted in a marked down-regulation (P=.004) of TLR4 at the protein level in the adipose tissue of challenged vs. control pigs, but LF and HF clearly blocked this response at the mRNA level. Although LF and HF also attenuated (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect