| Autor: |
Andressa Sbano, Lidiane Coelho Berbert, Rodolpho Mattos Albano, Vinícius Ribeiro Flores, João Victor Rego Ferreira, Ida Carolina Neves Direito, Janaína J. V. Cavalcante, Alexander M. Cardoso, Juliana Barbosa Succar |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Asian Journal of Biotechnology and Bioresource Technology. :11-13 |
| ISSN: |
2457-0125 |
| DOI: |
10.9734/ajb2t/2020/v6i130071 |
| Popis: |
Aims: This research aims to report the genome sequence of the Bacillus sp. strain AN2, isolated from agricultural soil from Rio de Janeiro, Brazil. Place and Duration of Study: Laboratory of Environmental Biotechnology, Western Rio Janeiro State University in Brazil, between January 2019 and December 2019. Methodology: High-quality genomic DNA was extracted using a GenElute Bacterial Genomic DNA kit. The Nextera XT DNA Library Prep Kit was used for genomic library construction. Paired-end sequence reads were generated by an Illumina MiSeq instrument with the 600 cycles MiSeq Reagent Kit v3. Sequence data were assembled with A5-MiSeq pipeline software and the contigs were annotated by Rapid Annotation using Subsystems Technology (RAST). Results: The obtained genome sequence of Bacillus AN2 included 21 contigs with a calculated size of 3,681,081 bp in length. The G + C content for the draft genome is 41.4%. A total of 3824 coding sequences (CDS) were predicted and encoded at least 88 tRNAs. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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