Determination of a novel photosensitizer sinoporphyrin sodium in human plasma by ultra-performance liquid chromatography-tandem mass spectrometry
| Autor: | Jia jia Yuan, Jun Zhou, Yun hai Bo, Lin Shen, Ji fang Gong, Fen Yang |
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| Rok vydání: | 2020 |
| Předmět: |
Bioanalysis
Analyte Spectrometry Mass Electrospray Ionization Porphyrins Formic acid Electrospray ionization Clinical Biochemistry Pharmaceutical Science Mass spectrometry 01 natural sciences High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Liquid chromatography–mass spectrometry Tandem Mass Spectrometry Drug Discovery Humans Photosensitizer Spectroscopy Chromatography High Pressure Liquid Chromatography Photosensitizing Agents 010405 organic chemistry Chemistry 010401 analytical chemistry Reproducibility of Results 0104 chemical sciences Chromatography Liquid |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 195 |
| ISSN: | 1873-264X |
| Popis: | Sinoporphyrin sodium (DVDMS) is a new photosensitizer (PS) and it is used in photodynamic therapy (PDT) against tumor. In this paper, a simple, rapid and specific ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the quantitation of DVDMS in human plasma was developed. The analytes were extracted from plasma samples using liquid-liquid extraction (LLE) after addition of testosterone (internal standard) and chromatographed on an AQUITY UPLC Protein BEH C4 column (50 × 2.1 mm, i.d. 1.7 μm) thermostatted at 40 °C with acetonitrile-water (0.1% formic acid and 0.1 mM K2EDTA) as the gradient mobile phase at flow rate of 0.5 mL/min. The detection was performed on an API 5500 mass spectrometer coupled with electrospray ionization (ESI) source in positive mode. The multiple reactions monitoring (MRM) transitions of m/z 1143.6→563.2 and m/z 289.3→109.1 were used to quantify DVDMS and IS, respectively. The assay was validated over the concentration range of 30−3000 ng/mL. Precision and accuracy are in accordance with the generally accepted criteria for bioanalytical methods. The extraction recovery and the matrix effect were investigated. This method was successfully applied to pharmacokinetic (PK) study of DVDMS in Chinese patients with solid tumor after treated with DVDMS-PDT for the first time. |
| Databáze: | OpenAIRE |
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