Immunochemistry of apamin--bee venom neurotoxin--I. Radioimmunoassay with apamin and its derivatives
| Autor: | Anatolij I. Miroshnikov, Elena A. Surina, Serguei V. Vasilenko, Serguei V. Komissarenko, Elena G. Elyakova |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Chemistry
musculoskeletal neural and ocular physiology Immunology Radioimmunoassay Pharmacology musculoskeletal system Inhibitory postsynaptic potential Apamin Arginine digestive system complex mixtures Antigen-Antibody Reactions chemistry.chemical_compound Bee Venoms Bee venom Immunochemistry Neurotoxin Animals Binding Sites Antibody Molecular Biology |
| Zdroj: | Molecular immunology. 18(6) |
| ISSN: | 0161-5890 |
| Popis: | Antibodies against apamin, a neurotoxic polypeptide from bee venom were raised in rabbits by immunization with apamin or apamin-BSA conjugates. 3 H-apamin or 125 I-apamin were used in radioimmunoassay with anti-apamin for the detection of the apamin antigenic site. The inhibitory activity toward the labelled apamin-anti-apamin binding was maximal with unlabelled apamin and decreased in the range: apamin > Cys 1 , Lys 4 -disuccinilated apamin > Cys 1 , Lys 4 -diacetylated apamin > Cys 1 , Lys 4 -diacetylated apamin with carboxymethylated His 18 . Dipyrimidyl-Orn 13 ,Orn 14 -apamin derivative almost had no inhibitory activity on labelled apamin binding emphasizing that Arg 13 , Arg 14 are the most essential for the apamin topographic antigenic site. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|