Abnormal effect of thiol groups on erythrocyte Na/Li countertransport kinetics in adult polycystic kidney disease
| Autor: | R. Wilkinson, T. H. Thomas, K. Vareesangthip |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Adult
Male medicine.medical_specialty Erythrocytes Adolescent Sodium Kinetics chemistry.chemical_element Lithium chemistry.chemical_compound Internal medicine medicine Humans Choline Spectrin Ion transporter Aged chemistry.chemical_classification Polycystic Kidney Diseases Transplantation business.industry Cell Membrane Sulfhydryl Reagents Middle Aged Membrane transport Red blood cell medicine.anatomical_structure Endocrinology chemistry Ethylmaleimide Nephrology Thiol Female business |
| Zdroj: | Nephrology Dialysis Transplantation. 10:2219-2223 |
| ISSN: | 1460-2385 0931-0509 |
| Popis: | Background. Evidence suggests that in adult polycystic kidney disease (APCKD) there is abnormal membrane organisation that involves cytoskeletal proteins and affects ion-transport proteins. The possibility of detecting a membrane organisation defect in erythrocytes from APCKD patients was investigated. Methods. Na/Li countertransport (CT) kinetics were measured in erythrocytes from APCKD patients compared with normal controls. The modulation of Na/Li CT kinetics by key membrane thiol groups and by dissociation of spectrin by heating was studied. Results. In erythrocytes from APCKD compared to normal subjects the Km of Na/Li CT was lower (64 SEM 3v 90 SEM 3mmol Na/l, P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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