Lipid-rich extract from Mexican avocado (Persea americana var. drymifolia) induces apoptosis and modulates the inflammatory response in Caco-2 human colon cancer cells
| Autor: | Lourdes Macías-Rodríguez, Marisol Báez-Magaña, Paul A. Spagnuolo, Mónica Lara-Márquez, Joel E. López-Meza, Rafael Salgado-Garciglia, Cristina Raymundo-Ramos, Alejandra Ochoa-Zarzosa |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Mitochondrial ROS Persea Medicine (miscellaneous) Apoptosis Pharmacology 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology Long-chain lipids Avocado medicine Cytotoxic T cell TX341-641 Caspase 030109 nutrition & dietetics Nutrition and Dietetics biology Nutrition. Foods and food supply Chemistry Superoxide Cancer 04 agricultural and veterinary sciences biology.organism_classification medicine.disease 040401 food science Colon cancer Caco-2 Persea americana biology.protein Food Science |
| Zdroj: | Journal of Functional Foods, Vol 64, Iss, Pp 103658-(2020) |
| ISSN: | 1756-4646 |
| DOI: | 10.1016/j.jff.2019.103658 |
| Popis: | Colorectal cancer (CRC) is the third most common type of cancer worldwide. Chemotherapy used in CRC patients has severe side effects. As an alternative, the search to identify natural compounds increases the opportunity to select therapeutics. In this work, the cytotoxic effect of a lipid extract (LEAS) from Mexican native avocado seed (Persea americana var. drymifolia) on colon cancer cells Caco-2 was determined. LEAS was cytotoxic towards Caco-2 cells (IC50 = 28 µg/ml), inducing apoptosis through the activation of caspases 8 and 9. Also, LEAS induced loss of membrane mitochondrial potential, inhibited fatty acid oxidation and increased the superoxide production and mitochondrial ROS. Furthermore, LEAS stimulated secretion of cytokines IL-6 (~500%), IL-8 (~400%) and IL-10 (~150%); whereas IL-1β secretion was inhibited (~50%). The results suggest that LEAS induces apoptosis on Caco-2 cells, indicating that avocado is a source of functional food products that can reduce the risk for development of cancer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect