Total Flavonoids Content in the Raw Material and Aqueous Extractives from Bauhinia monandra Kurz (Caesalpiniaceae)
| Autor: | Magda Rhayanny Assunção Ferreira, Tatiane Pereira de Souza, Luiz Alberto Lira Soares, Karina Perrelli Randau, Ana Josane Dantas Fernandes |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Coefficient of determination
Article Subject Stereochemistry Flavonoid lcsh:Medicine Raw material lcsh:Technology General Biochemistry Genetics and Molecular Biology lcsh:Science General Environmental Science chemistry.chemical_classification Flavonoids Chromatography Aqueous solution biology Chemistry Bauhinia lcsh:T Plant Extracts lcsh:R Water General Medicine Repeatability biology.organism_classification Solvent Bauhinia monandra lcsh:Q Research Article |
| Zdroj: | The Scientific World Journal The Scientific World Journal, Vol 2012 (2012) |
| ISSN: | 1537-744X 2356-6140 |
| Popis: | The aim of this work was to evaluate the spectrophotometric methodology for determining the total flavonoid content (TFC) in herbal drug and derived products fromBauhinia monandraKurz. Several analytical parameters from this method grounded on the complex formed between flavonoids and AlCl3were evaluated such as herbal amount (0.25 to 1.25 g); solvent composition (ethanol 40 to 80%, v/v); as well as the reaction time and AlCl3concentration (2 to 9%, w/v). The method was adjusted to aqueous extractives and its performance studied through precision, linearity and preliminary robustness. The results showed an important dependence of the method response from reaction time, AlCl3concentration, sample amount, and solvent mixture. After choosing the optimized condition, the method was applied for the matrixes (herbal material and extractives), showing precision lower than 5% (for both parameters repeatability and intermediate precision), coefficient of determination higher than 0.99, and no important influence could be observed for slight variations from wavelength or AlCl3concentration. Thus, it could be concluded that the evaluated analytical procedure was suitable to quantify the total flavonoid content in raw material and aqueous extractives from leaves ofB. monandra. |
| Databáze: | OpenAIRE |
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