The Chloroplast Ribonucleoprotein CP33B Quantitatively Binds the psbA mRNA
| Autor: | Jörg Nickelsen, Marlene Teubner, Kirsten Krause, Christian Schmitz-Linneweber, Hannes Ruwe, Lucas Bernal Espenberger, Benjamin Lenzen, Janina Fuss |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine rna recognition motif organelle rna processing VDP::Mathematics and natural science: 400::Basic biosciences: 470::Cell biology: 471 RNA-binding protein Plant Science arabidopsis thaliana 01 natural sciences 03 medical and health sciences psba rna-bind-n-seq d1 Organelle rna-binding Ecology Evolution Behavior and Systematics Ribonucleoprotein Messenger RNA Ecology RNA recognition motif Chemistry Botany RNA photosystem ii In vitro Cell biology Chloroplast 030104 developmental biology QK1-989 RNA Sequence VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Cellebiologi: 471 010606 plant biology & botany |
| Zdroj: | Plants, Vol 9, Iss 3, p 367 (2020) Plants Volume 9 Issue 3 |
| ISSN: | 2223-7747 |
| Popis: | Chloroplast RNAs are stabilized and processed by a multitude of nuclear-encoded RNA-binding proteins, often in response to external stimuli like light and temperature. A particularly interesting RNA-based regulation occurs with the psbA mRNA, which shows light-dependent translation. Recently, the chloroplast ribonucleoprotein CP33B was identified as a ligand of the psbA mRNA. We here characterized the interaction of CP33B with chloroplast RNAs in greater detail using a combination of RIP-chip, quantitative dot-blot, and RNA-Bind-n-Seq experiments. We demonstrate that CP33B prefers psbA over all other chloroplast RNAs and associates with the vast majority of the psbA transcript pool. The RNA sequence target motif, determined in vitro, does not fully explain CP33B&rsquo s preference for psbA, suggesting that there are other determinants of specificity in vivo. |
| Databáze: | OpenAIRE |
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