Production of neurospheres from mammalian Müller cells in culture

Autor: Claudine Versaux-Botteri, David Hicks, Germaine Michel, Julie Monnin, Nadège Morand-Villeneuve
Přispěvatelé: Laboratoire de Neurosciences Intégratives et Cliniques - UFC (EA 481) (NEURO), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université de Franche-Comté (UFC), Centre de Recherche des Cordeliers (CRC (UMR_S 872)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Ingénierie et biologie cellulaire et tissulaire (IBCT (ex IFR133)), Centre Hospitalier Régional Universitaire [Besançon] (CHRU Besançon)-Etablissement français du sang [Bourgogne-France-Comté] (EFS [Bourgogne-France-Comté])-Université de Franche-Comté (UFC), Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Université Paris Descartes - Paris 5 (UPD5)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté])-Université de Franche-Comté (UFC), Laboratoire de Neurosciences Intégratives et Cliniques - UFC (UR 481) (NEURO), Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS BFC)-Université de Franche-Comté (UFC), Laboratoire de Neurosciences Intégratives et Cliniques - UFC (EA 481) ( NEURO ), Université Bourgogne Franche-Comté [COMUE] ( UBFC ) -Université de Franche-Comté ( UFC ), Centre de Recherche des Cordeliers ( CRC (UMR_S 872) ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Ingénierie et biologie cellulaire et tissulaire ( IBCT (ex IFR133) ), Centre Hospitalier Régional Universitaire [Besançon] ( CHRU Besançon ) -Etablissement français du sang [Bourgogne-France-Comté] ( EFS [Bourgogne-France-Comté] ) -Université de Franche-Comté ( UFC ), Laboratoire de Neurosciences Intégratives et Cliniques - UFC ( NEURO ), Université Bourgogne Franche-Comté ( UBFC ) -Université de Franche-Comté ( UFC )
Rok vydání: 2007
Předmět:
MESH: Epidermal Growth Factor
MESH: Animals
Newborn
chemistry.chemical_compound
0302 clinical medicine
MESH: Glial Fibrillary Acidic Protein
MESH: Animals
MESH : Rats
Long-Evans
Cells
Cultured
MESH : Epidermal Growth Factor
0303 health sciences
Glial fibrillary acidic protein
biology
MESH : Rats
MESH : Animals
Newborn
MESH : Neuroglia
MESH: Retina
Stem Cells
General Neuroscience
MESH : Glial Fibrillary Acidic Protein
Cell biology
medicine.anatomical_structure
MESH : Stem Cells
Neuroglia
MESH: Neuroglia
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
MESH : Carrier Proteins
MESH: Cells
Cultured
MESH: Rats
MESH: Carrier Proteins
MESH: Stem Cells
Retina
03 medical and health sciences
MESH: Rats
Long-Evans
Neurosphere
MESH : Cells
Cultured
Glial Fibrillary Acidic Protein
medicine
Animals
Rats
Long-Evans
Progenitor cell
030304 developmental biology
Epidermal Growth Factor
MESH : Retina
Retinal
Nestin
In vitro
Culture Media
Rats
Animals
Newborn
chemistry
[ SDV.NEU ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
MESH: Culture Media
biology.protein
MESH : Culture Media
MESH : Animals
sense organs
Carrier Proteins
Neuroscience
030217 neurology & neurosurgery
Zdroj: Neuroscience Letters
Neuroscience Letters, Elsevier, 2007, 421 (1), pp.22-6. ⟨10.1016/j.neulet.2007.04.073⟩
Neuroscience Letters, 2007, 421 (1), pp.22-6. ⟨10.1016/j.neulet.2007.04.073⟩
Neuroscience Letters, Elsevier, 2007, 421 (1), pp.22-6. 〈10.1016/j.neulet.2007.04.073〉
ISSN: 0304-3940
DOI: 10.1016/j.neulet.2007.04.073
Popis: International audience; In lower vertebrates, like fishes and amphibians, retina is able to self-regenerate whereas Mammalian retina has lost this property. Nevertheless, recently, it has turned out that retinal glial Müller cells were playing a role in neuronal regeneration of the adult rodent retina, in case of acute damages, by dedifferentiating and redifferentiating in glial and neural cells. The purpose of this study was to analyse the ability of mammalian Müller cells for forming neurospheres. First of all, rats Müller cells were isolated in a primary culture. Second, these cells were resuspended in two different culture media: the cells cultures in the Neurobasal-A medium kept a typical Müller cells morphology even after 15 days of EGF treatment, and the cells plated in the DMEM-F12 medium formed neurospheres from the third day in culture. The neurosphere cells expressed nestin, cellular retinaldehyde binding protein (CRALBP) and glial fibrillary acidic protein (GFAP). These results showed our capacity to isolate and propagate Müller cells-derived progenitor cells. Moreover, it allows us to control the number of progenitor cells and, in the future, to study their differentiation capacity.
Databáze: OpenAIRE
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