Fibrin-alginate hydrogel supports steroidogenesis, in vitro maturation of oocytes and parthenotes production from caprine preantral follicles cultured in group
| Autor: | Cláudio Cabral Campello, R F Sousa, G.M. Silva, C.H. Lobo, Aaa Moura, Johan Smitz, Marcelo Bertolini, José Ricardo de Figueiredo, A.D. Sales, G.Q. Rodrigues, I.R. Brito, C. E. M. Calderón |
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| Přispěvatelé: | Follicle Biology |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Alginates Parthenogenesis Preantral follicle Fibrin Andrology 03 medical and health sciences Follicle 0302 clinical medicine Endocrinology Glucuronic Acid Ovarian Follicle Internal medicine medicine Animals fibrin oocyte Antrum 030219 obstetrics & reproductive medicine biology Chemistry Goats Hexuronic Acids Hydrogels Oocyte In vitro In Vitro Oocyte Maturation Techniques In vitro maturation 030104 developmental biology medicine.anatomical_structure IVM Oocytes biology.protein Female preantral follicle Animal Science and Zoology Alginate hydrogel Biotechnology |
| Zdroj: | Reproduction in Domestic Animals. 51:997-1009 |
| ISSN: | 0936-6768 |
| Popis: | This study aimed to establish a culture system that improves the in vitro development of caprine preantral follicles. In a first experiment, follicles were encapsulated as a single unit per bead and cultured singly or in groups or with five follicles in the same alginate (ALG) bead for 18 days. In a subsequent experiment, the "five follicles per bead" design was chosen to culture in ALG, fibrin-alginate (FA) or hyaluronate (HA) for 18 days. In a third experiment, we chose the five follicles per bead in FA to culture for 30 days. The culture set-up of five follicles per ALG bead increased antrum formation and follicle diameter compared to the other culture designs (p < .05). Moreover, under this condition, 44.44% of the oocytes from in vitro cultured preantral follicles reached meiotic resumption. A significant increase of follicle diameter occurred in attachment system and FA (p < .05), but the ALG condition reached the highest among all groups on day 18 (p < .05). Follicles encapsulated in matrix produced more estradiol and progesterone than attachment system (p < .05). The expression of MMP-9 mRNA was higher in FA than in other groups (p < .05) and similar to antral follicles from in vivo control (p > .05). Only FA group resulted in oocytes matured. After 30 days, oocytes from preantral follicles in vitro grown in FA developed to eight-cell parthenotes. In conclusion, a culture system using FA supported the development of caprine preantral follicles cultured in group and included in the same bead of hydrogel, improving the oocyte maturation and producing parthenotes. |
| Databáze: | OpenAIRE |
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