Fecal metabolite of a gnotobiotic mouse transplanted with gut microbiota from a patient with Alzheimer’s disease
| Autor: | Thuy Tien Thi Nguyen, Naotaka Kameya, Kensuke Arakawa, Shoji Nakamura, Yuta Fujimura, Yusuke Fujii, Hidetoshi Morita |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Time Factors Metabolite Disease Biology Gut flora Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry Microbiology Pathogenesis Feces Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Alzheimer Disease Metabolome Animals Germ-Free Life Humans Metabolomics Molecular Biology Behavior Animal Organic Chemistry General Medicine biology.organism_classification Gastrointestinal Microbiome Transgenesis Disease Models Animal 030104 developmental biology chemistry 16s rrna gene sequencing 030217 neurology & neurosurgery Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 83:2144-2152 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1080/09168451.2019.1644149 |
| Popis: | Studies of Alzheimer’s disease are based on model mice that have been altered by transgenesis and other techniques to elicit pathogenesis. However, changes in the gut microbiota were recently suggested to diminish cognitive function in patients, as well as in model mice. Accordingly, we have created model mice of the human gut microbiota by transplanting germ-free C57BL/6N mice with fecal samples from a healthy volunteer and from an affected patient. These humanized mice were stably colonized and reproduced the bacterial diversity in donors. Remarkably, performance on Object Location Test and Object Recognition Test was significantly reduced in the latter than in the former at 55 weeks of age, suggesting that gut microbiota transplanted from an affected patient affects mouse behavior. In addition, metabolites related to the nervous system, including γ-aminobutyrate, taurine, and valine, were significantly less abundant in the feces of mice transplanted with microbiota from the affected patient. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|