Application of liquid chromatography-electrospray ionization mass spectrometry for study of steroid-converting enzymes
| Autor: | Zdeněk Deyl, Kateřina Mikulíková, Marek Kučka, Jiří Pácha, Ivan Mikšík |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Electrospray Spectrometry Mass Electrospray Ionization medicine.medical_treatment Electrospray ionization Clinical Biochemistry Guinea Pigs Oviducts Mass spectrometry Biochemistry Sample preparation in mass spectrometry Analytical Chemistry Steroid Liquid chromatography–mass spectrometry medicine Animals Hydroxysteroid dehydrogenase Rats Wistar Chromatography High Pressure Liquid Chromatography Chemistry Elution Cell Biology General Medicine Enzymes Rats Intestines Calibration 11-beta-Hydroxysteroid Dehydrogenases Female Indicators and Reagents Spectrophotometry Ultraviolet Steroids Corticosterone Chickens |
| Zdroj: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 800(1-2) |
| ISSN: | 1570-0232 |
| Popis: | A high-performance liquid chromatography–atmospheric pressure ionization–electrospray ionization mass spectrometry (HPLC–API– ESI–MS) method was developed for the analysis of steroids in a study of steroid-converting enzymes. Separations ware done on a Zorbax Eclipse XDB-C18 column (eluted with a linear methanol–water–acetic acid gradient) and identification of the steroids involved was done by API–ESI–MS using positive ion mode and extracted ion analysis. The applicability of the present method for studying steroid metabolism was proven in assaying two steroid-converting enzymes (20- hydroxysteroid dehydrogenase and 11-hydroxysteroid dehydrogenase) in various biological samples (rat and chicken intestine, chicken oviduct). © 2003 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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