Low concentration of oleic acid exacerbates LPS-induced cell death and inflammation in human alveolar epithelial cells

Autor:	Altug Kucukgul, Suat Erdogan
Rok vydání:	2017
Předmět:	0301 basic medicine Pulmonary and Respiratory Medicine Lipopolysaccharides Programmed cell death Lipopolysaccharide Clinical Biochemistry Acute Lung Injury Inflammation Lung injury Biology 03 medical and health sciences chemistry.chemical_compound medicine Humans Molecular Biology Volume concentration Cell Death respiratory system In vitro respiratory tract diseases Oleic acid Oxidative Stress 030104 developmental biology chemistry Apoptosis A549 Cells Alveolar Epithelial Cells Immunology Cancer research lipids (amino acids peptides and proteins) medicine.symptom Oleic Acid
Zdroj:	Experimental lung research. 43(1)
ISSN:	1521-0499
Popis:	The current study aimed to investigate in vitro effects of oleic acid on lipopolysaccharide (LPS)-induced acute lung injury in the human lung epithelial cells (A549).The cell viability was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) tests. Selected gene expression levels were analyzed by Real-Time Quantitative-Polymerase Chain Reaction (RT-qPCR).24 hours of LPS (100 ng/mL) exposure decreased the cells' viability by 44.6% compared to untreated control. Low concentration (2.5 nM) of oleic acid slightly suppressed the cell survival by 9.1% analyzed 24 hours after incubation. However, oleic acid pretreatment before LPS exposure significantly increased cell survival loss to 63.9%. LPS exposure decreased the expressions of catalase (CAT) and glutathione peroxidase (GPx) mRNA levels by 2.8 and 2.5 fold, respectively. Moreover, pretreatment of the cells with oleic acid strengthened LPS-decreased expressions of CAT and GPx genes by 3.5 and 6.7 fold, respectively. The mRNA expressions of superoxide dismutase (SOD), induced nitric oxide synthase (iNOS), interleukin-1β, IL-12, COX-2, caspase-3 and caspase-8 were increased by 2.4, 2.2, 2.2, 2.3, 3.0, 2.6, and 2.5 fold, respectively, by LPS, and oleic acid pretreatment significantly potentiated the effect of LPS.Oleic acid worsens LPS-induced cell death by potentiating oxidative stress and inflammation in A549 lung epithelial cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1c5d1cf60b78828b9a8a31fa715582af https://pubmed.ncbi.nlm.nih.gov/28080141 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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