Apoptin induces apoptosis in human transformed and malignant cells but not in normal cells
| Autor: | D. F. Fischer, B. Klein, Claude Backendorf, Paul H.A. Quax, J. H. F. Falkenburg, Mathieu H.M. Noteborn, Jos M. Grimbergen, A. A. A. M. Danen-van Oorschot, Shi-Mei Zhuang, A. J. Van Der Eb |
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| Rok vydání: | 1997 |
| Předmět: |
Male
Programmed cell death T-Lymphocytes Apoptosis Simian virus 40 Biology Transfection law.invention Capsid law Skin Physiological Phenomena Tumor Cells Cultured medicine Humans Fluorescent Antibody Technique Indirect Cells Cultured Cell Line Transformed Skin Multidisciplinary Fibroblasts Biological Sciences Recombinant Proteins In vitro Cell biology Cell Transformation Neoplastic medicine.anatomical_structure Cytoplasm Recombinant DNA Capsid Proteins Nucleus Chicken anemia virus |
| Zdroj: | Proceedings of the National Academy of Sciences. 94:5843-5847 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | The chicken anemia virus protein apoptin induces a p53-independent, Bcl-2-insensitive type of apoptosis in various human tumor cells. Here, we show that, in vitro , apoptin fails to induce programmed cell death in normal lymphoid, dermal, epidermal, endothelial, and smooth-muscle cells. However, when normal cells are transformed they become susceptible to apoptosis by apoptin. Long-term expression of apoptin in normal human fibroblasts revealed that apoptin has no toxic or transforming activity in these cells. In normal cells, apoptin was found predominantly in the cytoplasm, whereas in transformed and malignant cells it was located in the nucleus, suggesting that the localization of apoptin is related to its activity. These properties make apoptin a potential agent for the treatment of a large number of tumors, also those lacking p53 and/or overexpressing Bcl-2. |
| Databáze: | OpenAIRE |
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