Danggui Buxue Tang, a simple Chinese formula containing Astragali Radix and Angelicae Sinensis Radix, stimulates the expressions of neurotrophic factors in cultured SH-SY5Y cells
| Autor: | Karl Wah Keung Tsim, Amy G. W. Gong, Huai Y Wang, Yuzhong Zheng, Tina T. X. Dong |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pharmacology MAPK/ERK pathway SH-SY5Y biology Traditional medicine Neuronal functions business.industry Research Danggui Buxue Tang CREB medicine.disease SH-SY5Y cells 03 medical and health sciences 030104 developmental biology Nerve growth factor Complementary and alternative medicine Neurotrophic factors Neuroblastoma biology.protein medicine Glial cell line-derived neurotrophic factor business Neurotrophin |
| Zdroj: | Chinese Medicine |
| ISSN: | 1749-8546 |
| Popis: | Background Danggui Buxue Tang (DBT), a phytoestrogen-enriched Chinese herbal formula, serves as dietary supplement in stimulating the “Blood” functions of menopausal women. In traditional Chinese medicine (TCM) theory, “Blood” has a strong relationship with brain activities. Previous studies supported that some ingredients of DBT possessed neuronal beneficial functions. Therefore, the neurotrophic function and the mechanistic action of DBT were systematically evaluated in cultured human neuroblastoma SH-SY5Y cells. Methods The DBT-triggered protein expressions were analyzed by western blotting, while the transcriptional activities of promoters coding for related genes were revealed by luciferase assays. For mechanistic analysis of DBT, Erk1/2 and its inhibitor U0126 were analyzed. Results The application of DBT in cultured neuroblastoma cells showed the efficacies in: (1) up-regulation of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF); (2) activation of transcriptional activities of promoters coding for NGF, BDNF, GDNF; (3) activation of Erk1/2 and CREB; and (4) attenuation of the neurotrophic factor expression by the treatment of an Erk1/2 inhibitor. Conclusions Our study supports that MAPK/Erk pathway acts as fundamental role in monitoring DBT-induced expression of neurotrophic factors in cultured human neuroblastoma cell. These results shed light in developing the working mechanism of this ancient herbal decoction for its neuronal function. Electronic supplementary material The online version of this article (doi:10.1186/s13020-017-0144-y) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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