Intraperitoneal radio-localization of tumors pre-targeted by biotinylated monoclonal antibodies
| Autor: | Massimo Malcovati, Agamemnon A. Epenetos, G. A. Scassellati, G. Paganelli, Shahid Pervez, G. Deleide, G. Rowlinson, Antonio G. Siccardi, F. Chiolerio |
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| Rok vydání: | 1990 |
| Předmět: |
Streptavidin
Cancer Research Time Factors medicine.drug_class Transplantation Heterologous Drug Evaluation Preclinical Biotin Mice Nude Monoclonal antibody Cell Line Iodine Radioisotopes chemistry.chemical_compound Mice Peritoneum Bacterial Proteins medicine Animals Radionuclide Imaging Peritoneal Neoplasms biology Indium Radioisotopes Antibodies Monoclonal Avidin Molecular biology Immunohistochemistry medicine.anatomical_structure Oncology chemistry Biotinylation Isotope Labeling biology.protein Autoradiography Antibody Neoplasm Transplantation |
| Zdroj: | International journal of cancer. 45(6) |
| ISSN: | 0020-7136 |
| Popis: | We describe 2-step and 3-step strategies for intraperitoneal tumor radio-localization by means of monoclonal antibodies (MAbs). Nude mice bearing intraperitoneal human colon carcinoma tumors were injected i.p. with biotinylated MAb AUAI, followed 24 hr later by radioiodinated streptavidin (2-step). The uptake of radioactivity in tumor and normal tissues was measured 4 hr after injection of radioactive compound. A 3-step strategy consisted in administering biotinylated antibody, cold avidin after 24 hr and 111In-labelled biotin after a further 4 hr; mice were then killed 2 hr later. Tumor localization of intraperitoneally-administered biotinylated antibody and direct targeting of radioactive streptavidin to biotinylated antibody bound to tumor sites were demonstrated using immunohistochemistry and autoradiography. Our results show that (i) the 2-step approach increased the percentage of radioactivity uptake by tumor with respect to directly labelled antibodies (24% vs. 6%) and improved the tumor/non-tumor ratio; (ii) the 3-step approach allowed faster blood clearance of the radioactive probe (111In-biotin) and yielded high tumor/non-tumor ratios. "Pre-targeting" methods appear to have advantages over the conventional 1-step approach with directly radiolabelled antibody. |
| Databáze: | OpenAIRE |
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