Evaluation of a commercial enzyme-linked immunosorbent assay for Johne's disease
| Autor: | Michael T. Collins, J C Cox, Ridge Se, D C Sockett |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Microbiology (medical)
Veterinary medicine Paratuberculosis Enzyme-Linked Immunosorbent Assay Biology Sensitivity and Specificity Microbiology Mycobacterium medicine Animals Dairy cattle Feces Subclinical infection Bacteriological Techniques medicine.diagnostic_test Complement fixation test medicine.disease Antibodies Bacterial Diarrhea Evaluation Studies as Topic Immunoassay Herd Cattle medicine.symptom Research Article |
| Zdroj: | Journal of clinical microbiology. 29(2) |
| ISSN: | 0095-1137 |
| Popis: | A new commercial kit for diagnosis of bovine paratuberculosis (Johne's disease), called the Johne's Absorbed EIA (enzyme immunoassay; Commonwealth Serum Laboratories, Parkville, Victoria, Australia), was evaluated by using serum specimens from the National Repository for Paratuberculosis Specimens. The evaluation was specifically designed to measure test sensitivity and specificity for detection of dairy cattle with subclinical paratuberculosis. The case definition of subclinical bovine paratuberculosis was isolation of Mycobacterium paratuberculosis from fecal samples or internal organs of cattle without diarrhea or chronic weight loss. Animals designed as free of the disease originated exclusively from four herds in Wisconsin that were certified to be free of disease. The kit had a sensitivity of 47.3% for serum specimens from 150 infected cattle. The test detected 59.7% of animals that shed M. paratuberculosis in their feces, as defined by conventional fecal culture, at the time of serum collection. Testing of 196 serum specimens from cattle without paratuberculosis yielded two false-positive results; the test specificity was thus 99.0%. Decision analysis procedures on the economics of using the kit in a test-and-cull disease control program indicated it would be cost-effective in any herd with a true paratuberculosis prevalence of greater than or equal to 3%. Comparison of the sensitivity and specificity of the Johne's Absorbed EIA with those of other tests for detection of subclinical paratuberculosis indicated that it may be the most accurate commercially available test at present and better than standard complement fixation test used in the United States. |
| Databáze: | OpenAIRE |
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