Lysosomal Proteolysis Is Associated With Exercise-Induced Improvement of Mitochondrial Quality Control in Aged Hippocampus
| Autor: | Jia-Ru Dai, Shan-Shan Guo, Zheng-Hong Qin, A-Ming Lu, Xiao-Fang Gao, Yan Wang, Zhou Zhu, Lisa Wood, Haidong Xu, Li Luo, Xiao-Fei Zhang, Yan-Rong Gu |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Aging medicine.medical_specialty Immunoblotting Hippocampus Fluorescent Antibody Technique Mitochondrion medicine.disease_cause Real-Time Polymerase Chain Reaction Thiobarbituric Acid Reactive Substances Rats Sprague-Dawley 03 medical and health sciences Microscopy Electron Transmission Internal medicine Physical Conditioning Animal Mitophagy medicine Autophagy Animals Cognitive decline Organelle Biogenesis business.industry Rats Oxidative Stress 030104 developmental biology Endocrinology Biochemistry Mitochondrial biogenesis mitochondrial fusion Proteolysis Geriatrics and Gerontology business Lysosomes Oxidative stress |
| Zdroj: | The journals of gerontology. Series A, Biological sciences and medical sciences. 72(10) |
| ISSN: | 1758-535X |
| Popis: | Exercise improves cognitive function in older adults, but the underlying mechanism is largely unknown. Both lysosomal degradation and mitochondrial quality control decline with age. We hypothesized that exercise ameliorates age-related cognitive decline through the improvement of mitochondrial quality control in aged hippocampus, and this effect is associated with lysosomal proteolysis. Sixteen to eighteen-month old male Sprague Dawley rats underwent swim exercise training for 10 weeks. The exercise regimen prevented cognitive decline in aged rats, reduced oxidative stress, and rejuvenated mitochondria in the aged hippocampus. Exercise training promoted mitochondrial biogenesis, increased mitochondrial fusion and fission, and activated autophagy/mitophagy in aged hippocampal neurons. Lysosomal inhibitor chloroquine partly blocked beneficial effects of exercise on cognitive function, oxidative stress, autophagy/mitophagy, and mitochondrial quality control in aged rats. These results suggest that preservation of cognitive function by long-term exercise is associated with improvement of mitochondrial quality control in aged hippocampus and that lysosomal degradation is required for this process. Our findings suggest that exercise training or pharmacological regulation of mitochondrial quality control and lysosomal degradation may be effective strategies for slowing down age-related cognitive decline. |
| Databáze: | OpenAIRE |
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