Age-Related Changes in O‑Acetylation of Sialic Acids Bound to N‑Glycans of Male Rat Serum Glycoproteins and Influence of Dietary Intake on Their Changes
| Autor: | Shigeo Suzuki, Mitsuhiro Kinoshita, Sachio Yamamoto |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
chemistry.chemical_classification
Glycan Fatty acid metabolism biology Chemistry General Chemical Engineering General Chemistry Article Food restriction carbohydrates (lipids) chemistry.chemical_compound Capillary electrophoresis Biochemistry Acetylation biology.protein Glycolysis Glycoprotein Relative species abundance QD1-999 |
| Zdroj: | ACS Omega, Vol 5, Iss 30, Pp 18608-18618 (2020) ACS Omega |
| ISSN: | 2470-1343 |
| Popis: | O-Acetylation of sialic acids has been widely found in eukaryotic cells. Such modifications of sialic acids are tissue-specific and seem to be developmentally regulated. In this study, we performed comprehensive analysis of age-related changes in the serum N-glycans of male rats using capillary electrophoresis (CE) and investigated the changes in the O-acetylation of sialic acids bound to N-glycans with aging and different diets. The present method offered sufficient resolution to assess the degree of O-acetylation of the N-glycans and allowed for the determination of the age-related changes in O-acetylation of sialic acids. Using the CE-based method, we found that the relative abundance of disialo-biantennary N-glycans modified with 9-O-acetylated N-acetylneuraminic acid (Neu5,9Ac) significantly increased with aging. In addition, the relative abundances of N-glycans with two Neu5,9Ac reversed to those of N-glycans with only Neu5Ac during 12 weeks. Next, we evaluated the influence of high-fat diet and food restriction on age-related changes in O-acetylation. Although the total amount of disialo-biantennary N-glycans increased with aging, age-related O-acetylation of sialic acids was suppressed by a high-fat diet. On the other hand, food restriction enhanced the O-acetylation of sialic acids, and the relative abundance of N-glycans with two Neu5,9Ac residues at 15 weeks of age was higher than that observed in the standard diet group. These findings suggest that the O-acetylation of sialic acids is closely related to changes in energy metabolisms such as glycolysis or fatty acid metabolism. |
| Databáze: | OpenAIRE |
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