Proteoglycan changes in carcinogen (4NQO)-treated rat tongue mucosa
| Autor: | Allan Vreugdenburg, D. F. Wilson, Ole W. Wiebkin |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Administration Topical Connective tissue Epithelium Pathology and Forensic Medicine Glycosaminoglycan Extracellular matrix Body Water Tongue medicine Extracellular Animals Neoplasm Invasiveness Carcinogen Glycosaminoglycans Extracellular Matrix Proteins Hyperplasia biology Carcinoma Mouth Mucosa Rats Inbred Strains Molecular biology 4-Nitroquinoline-1-oxide Rats Tongue Neoplasms medicine.anatomical_structure Otorhinolaryngology Proteoglycan Solubility Connective Tissue biology.protein Periodontics Immunohistochemistry Female Proteoglycans Oral Surgery Precancerous Conditions |
| Zdroj: | Journal of oral pathologymedicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology. 24(3) |
| ISSN: | 0904-2512 |
| Popis: | The purpose of this study was to undertake preliminary analyses of the extracellular proteoglycans in carcinogen [4-nitroquinoline N-oxide (4NQO)]-treated rat tongue mucosa. Experimental rats were exposed to twice-weekly applications of 4NQO in propylene glycol for six months, after which the animals were killed. Control and 4NQO-treated tissues were subjected to sequential aqueous extractions of proteoglycans under associative and dissociative conditions, followed by alkaline cleavage of protein-glycosaminoglycan linkages to yield a glycosaminoglycan residue. Tissues subjected to 4NQO applications contained smaller proportions of proteoglycans which were readily soluble under associative and dissociative conditions. Proportionately more proteoglycan remained strongly associated with other intercellular tissue components, being released only by alkaline cleavage. These biochemical alterations in preinvasive 4NQO-treated epithelium and connective tissues, together with an observed associated change in water retention by the connective tissue, occurred prior to actual neoplastic invasion and suggest differences in macromolecular conformation and orderliness. We hypothesize that these changes are related to the phenomenon of neoplastic epithelial invasion. |
| Databáze: | OpenAIRE |
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