Biomarkers of early stage osteoarthritis, rheumatoid arthritis and musculoskeletal health
| Autor: | Naila Rabbani, Attia Anwar, Usman Ahmed, Matthew L. Costa, Nicola D Mackay, Paul G. Winyard, Paul J. Thornalley, Andrew Filer, Richard S. Savage, Richard C Haigh, Karim Raza, Joanna M. Tarr, Richard A. Watts |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Arthritis Osteoarthritis medicine.disease_cause Sensitivity and Specificity Article Autoimmunity Arthritis Rheumatoid chemistry.chemical_compound Tandem Mass Spectrometry medicine Citrulline Humans Rheumatoid factor Musculoskeletal Diseases Chromatography High Pressure Liquid Aged Autoantibodies Multidisciplinary biology business.industry Autoantibody Middle Aged medicine.disease Hydroxyproline Early Diagnosis ROC Curve chemistry Area Under Curve Rheumatoid arthritis Immunology biology.protein Female Antibody business Algorithms Biomarkers |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep09259 |
| Popis: | There is currently no biochemical test for detection of early-stage osteoarthritis (eOA). Tests for early-stage rheumatoid arthritis (eRA) such as rheumatoid factor (RF) and anti–cyclic citrullinated peptide (CCP) antibodies require refinement to improve clinical utility. We developed robust mass spectrometric methods to quantify citrullinated protein (CP) and free hydroxyproline in body fluids. We detected CP in the plasma of healthy subjects and surprisingly found that CP was increased in both patients with eOA and eRA whereas anti–CCP antibodies were predominantly present in eRA. A 4-class diagnostic algorithm combining plasma/serum CP, anti-CCP antibody and hydroxyproline applied to a cohort gave specific and sensitive detection and discrimination of eOA, eRA, other non-RA inflammatory joint diseases and good skeletal health. This provides a first-in-class plasma/serum-based biochemical assay for diagnosis and type discrimination of early-stage arthritis to facilitate improved treatment and patient outcomes, exploiting citrullinated protein and related differential autoimmunity. |
| Databáze: | OpenAIRE |
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