Mouth Washing Impaired SARS-CoV-2 Detection in Saliva
| Autor: | Nicolas Benoit, Lionel Almeras, Bruno Pradines, Hervé Tissot-Dupont, Monique Melo Costa, Samuel Granjeaud, Matthieu Million |
|---|---|
| Přispěvatelé: | Vecteurs - Infections tropicales et méditerranéennes (VITROME), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées (IRBA), Institut de Recherche Biomédicale des Armées [Antenne Marseille] (IRBA), Microbes évolution phylogénie et infections (MEPHI), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche en Cancérologie de Marseille (CRCM), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Aix Marseille Université (AMU), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] (IRBA), Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), COMBE, Isabelle |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Medicine (General)
COVID-19 diagnosis Saliva medicine.medical_specialty Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Clinical Biochemistry coronavirus Saliva sample Gastroenterology Asymptomatic Article 03 medical and health sciences R5-920 0302 clinical medicine [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system Paired samples stomatognathic system [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases Saliva testing Internal medicine medicine [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology 030212 general & internal medicine [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology [SDV.MHEP.ME] Life Sciences [q-bio]/Human health and pathology/Emerging diseases saliva 0303 health sciences [SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases SARS-CoV-2 030306 microbiology business.industry allergology [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [SDV.MHEP.CSC] Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system Homogeneous [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology medicine.symptom business Viral load [SDV.MP.PAR] Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology |
| Zdroj: | Diagnostics Diagnostics, MDPI, 2021, 11 (8), pp.1509. ⟨10.3390/diagnostics11081509⟩ Diagnostics, 2021, 11 (8), pp.1509. ⟨10.3390/diagnostics11081509⟩ Volume 11 Issue 8 Diagnostics, Vol 11, Iss 1509, p 1509 (2021) |
| ISSN: | 2075-4418 |
| Popis: | Background: A previous study demonstrated the performance of the Salivette® (SARSTEDT, Numbrecht, Germany) as a homogeneous saliva collection system to diagnose COVID-19 by RT-qPCR, notably for symptomatic and asymptomatic patients. However, for convalescent patients, the corroboration of molecular detection of SARS-CoV-2 in paired nasopharyngeal swabs (NPS) and saliva samples was unsatisfactory. Objectives: The aim of the present work was to assess the concordance level of SARS-CoV-2 detection between paired sampling of NPSs and saliva collected with Salivette® at two time points, with ten days of interval. Results: A total of 319 paired samples from 145 outpatients (OP) and 51 healthcare workers (HW) were collected. Unfortunately, at day ten, 73 individuals were lost to follow-up, explaining some kinetic missing data. Due to significant waiting rates at hospitals, most of the patients ate and/or drank while waiting for their turn. Consequently, mouth washing was systematically proposed prior to saliva collection. None of the HW were diagnosed as SARS-CoV-2 positive using NPS or saliva specimens at both time points (n = 95) by RT-qPCR. The virus was detected in 56.3% (n = 126/224) of the NPS samples from OP, but solely 26.8% (n = 60/224) of the paired saliva specimens. The detection of the internal cellular control, the human RNase P, in more than 98% of the saliva samples, underlined that the low sensitivity of saliva specimens (45.2%) for SARS-CoV-2 detection was not attributed to an improper saliva sample storing or RNA extraction. Conclusions: This work revealed that mouth washing decreased viral load of buccal cavity conducting to impairment of SARS-CoV-2 detection. Viral loads in saliva neo-produced appeared insufficient for molecular detection of SARS-CoV-2. At the time when saliva tests could be a rapid, simple and non-invasive strategy to assess large scale schoolchildren in France, the determination of the performance of saliva collection becomes imperative to standardize procedures. |
| Databáze: | OpenAIRE |
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