Antimicrobial activities of culturable microorganisms (actinomycetes and fungi) isolated from Chaabe Cave, Algeria
| Autor: | Larbi Belyagoubi, Nabila Belyagoubi-Benhammou, Djamel Eddine Abdelouahid, Souad Benaissa, Valme Jurado, Fatima Zohra Djebbah, Sandrine Lacoste, Joëlle Dupont, Salim Habi, Cesáreo Sáiz-Jiménez, Fatima Z. Ounadjela |
|---|---|
| Přispěvatelé: | Jurado, Valme, Sáiz-Jiménez, Cesáreo, Jurado, Valme [0000-0003-0972-9909], Sáiz-Jiménez, Cesáreo [0000-0003-0036-670X] |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
QE1-996.5 geography geography.geographical_feature_category biology QH301-705.5 Microorganism Penicillium Geology Antimicrobial activity Cave microorganisms Antimicrobial biology.organism_classification Streptomyces 03 medical and health sciences 030104 developmental biology Cave Botany antimicrobial activities Chaabe Cave Biology (General) Earth-Surface Processes |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname International Journal of Speleology, Vol 47, Iss 2, Pp 189-199 (2018) |
| ISSN: | 1827-806X 0392-6672 |
| DOI: | 10.5038/1827-806x.47.2.2148 |
| Popis: | 11 páginas.-- 2 figuras.-- 6 tablas.-- 65 referencias The discovery of new antibiotics and other bioactive microbial metabolites is a priority given the frequency of the emerging multi-drug resistant pathogenic microorganisms. Thus, scientists are searching for new antibiotics in microorganisms isolated from extreme habitats such as caves. In the present study, we aimed to perform the first analysis on culturable microorganisms in the Chaabe Cave (Algeria), and to test the antimicrobial activities of the isolates (Streptomyces spp. and Penicillium spp.). The potential for antimicrobial activity of 47 strains of actinomycetes and 23 strains of fungi were tested on Candida albicans, Staphylococcus aureus, Micrococcus luteus, Listeria monocytogenes, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa. The identification of the most active actinomycetes strains by 16S rRNA gene sequence analysis revealed that these isolates were exclusively affiliated to the genus Streptomyces. On the other hand, the fungi were determined by phylogenetic analysis based on the ITS region or on the β tubulin gene and were affiliated to the genera Readeriella, Cladosporium, Aspergillus, Penicillium, Beauveria, Alternaria, Rhizopus, and Rhizomucor. The strains showed strong inhibitory activity against pathogenic microorganisms and the diameters of the inhibition zones vary between 7.5 and 34 mm for Streptomyces strains, and between 6.5 and 19.50 mm for Penicillium strains. The data indicated that the majority (72.86%) of the 70 isolates were active against at least one of the tested microorganisms. The production of nonpolyenic antifungal substances by active Streptomyces isolates was investigated using several criteria (antibacterial activity, ergosterol inhibition, and UV-visible spectra) of active extracts. The results were promising and showed that the metabolites produced by the actinomycete strains do not have a UV-visible spectrum characteristic of a polyenic structure. The Chaabe Cave possesses a diversity of microorganisms that could lead to new antibiotics necessary in the fight against drug-resistant pathogens and warrant further study |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|