Effect of Heteropterys aphrodisiaca on Anxiety and Male Exposure of Female Mice with Advanced Age
| Autor: | André Barciela Veras, Rozimeri Colleti, Susana Elisa Moreno, Sergio A.S. Machado, Eric Murillo-Rodríguez, Daniela Miranda Farias, Maria Silvia Ostetto, Jorge Aparecido Barros, Thais Barbosa |
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| Rok vydání: | 2018 |
| Předmět: |
Male
Aging medicine.medical_specialty Time Factors medicine.drug_class Anxiety Placebo Anxiolytic Mice Escape Reaction Internal medicine Avoidance Learning medicine Animals Testosterone Pharmacology (medical) Aphrodisiac General Pharmacology Toxicology and Pharmaceutics Heteropterys Behavior Animal biology Plant Extracts business.industry Liter General Medicine biology.organism_classification Disease Models Animal Endocrinology Anti-Anxiety Agents Female medicine.symptom business Malpighiaceae Hormone |
| Zdroj: | Current Clinical Pharmacology. 12:106-112 |
| ISSN: | 1574-8847 |
| Popis: | BACKGROUND Effect of Heteropterys aphrodisiaca (dog-node) on anxiety and function of adult female wistar mice. The project is an experiment with the use of H. aphrodisiac root extract, in order to observe the frequency of sexual exposure of females exposed to the extract, quantify the effect of the extract on the concentration of total testosterone and observe the anxiety levels of the animals exposed. Results will be measured with the laboratory testosterone test and LCE and CA tests. METHODS In preparation of the extract, the root was oven dried at 40°C and diluted in alcohol extract (100g of root for 1 liter of alcohol) and lyophilized. 40 adult female mice were enrolled, separated in control group (placebo) and treatment group (50 mg/kg/day) for 15, 30, 45 and 60 days. At each period, hormonal testosterone and anxiety levels by the Elevated-Cross Labyrinth (ECL) tests and Open Camp (CA) were measured in 10 animals that were later euthanized (SBNeC). RESULTS The results showed an improvement in the decrease of anxiety, as shown in the variables of number of open arm entries, time on the same side of the field, less avoidance and leakage. However, it appears that the time of exposure to the extract does not result in increased benefit, with possible decline of effect after 45 days of use. CONCLUSION With this performed experiment with the "no-de-cachorro" extract, it was possible to understand a little more how this root can act in relation to anxiety, as predicted by the pharmacology that validates the animal models; anxiolytic components decrease anxiety-related behaviors, as shown in the variables of entry numbers in the open arm, time on the same side of the field, less avoidance and escape. However, it seems that the time of exposure to the extract does not modify the performance in the tests, observing until an apparent exhaustion of the anxiolytic action, which evidences the need for more specific studies on the possible effects of the extract. |
| Databáze: | OpenAIRE |
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