Circulating microRNAs as stable blood-based markers for cancer detection
| Autor: | Peter S. Nelson, Patrick S. Mitchell, Stacia K. Wyman, Charles W. Drescher, Amelia Peterson, Rachael K. Parkin, April Allen, Robert L. Vessella, Daniel W. Lin, Kathy O'Briant, Muneesh Tewari, Daniel Martin, Evan M. Kroh, Robert Gentleman, Brian R. Fritz, Era L. Pogosova-Agadjanyan, Beatrice S. Knudsen, Nicole Urban, Derek L. Stirewalt, Jennifer Noteboom |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Male
PCA3 Biology Bioinformatics Sensitivity and Specificity Mice Prostate cancer Ribonucleases Neoplasms microRNA Biomarkers Tumor medicine Animals Humans RNA Neoplasm Letters Cloning Molecular Multidisciplinary Gene Expression Profiling Prostatic Neoplasms Cancer Biological Sciences medicine.disease Gene Expression Regulation Neoplastic Gene expression profiling MicroRNAs Circulating MicroRNA Cancer research Biomarker (medicine) Neoplasm Transplantation Extracellular RNA |
| Zdroj: | Proceedings of the National Academy of Sciences. 105:10513-10518 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.0804549105 |
| Popis: | Improved approaches for the detection of common epithelial malignancies are urgently needed to reduce the worldwide morbidity and mortality caused by cancer. MicroRNAs (miRNAs) are small (≈22 nt) regulatory RNAs that are frequently dysregulated in cancer and have shown promise as tissue-based markers for cancer classification and prognostication. We show here that miRNAs are present in human plasma in a remarkably stable form that is protected from endogenous RNase activity. miRNAs originating from human prostate cancer xenografts enter the circulation, are readily measured in plasma, and can robustly distinguish xenografted mice from controls. This concept extends to cancer in humans, where serum levels of miR-141 (a miRNA expressed in prostate cancer) can distinguish patients with prostate cancer from healthy controls. Our results establish the measurement of tumor-derived miRNAs in serum or plasma as an important approach for the blood-based detection of human cancer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|