Denatonium and Naringenin Promote SCA-9 Tumor Growth and Angiogenesis: Participation of Arginase
| Autor: | Maria Ester Castro, María Elena Sales, Ganna Dmytrenko |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Naringenin Male Vascular Endothelial Growth Factor A Cancer Research medicine.medical_specialty Angiogenesis Medicine (miscellaneous) Nitric Oxide Nitric oxide Receptors G-Protein-Coupled 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine stomatognathic system GTP-Binding Proteins Internal medicine Cell Line Tumor medicine Animals Urea Cell Proliferation Nutrition and Dietetics biology Arginase Dose-Response Relationship Drug Neovascularization Pathologic Denatonium Submandibular gland Cell biology Nitric oxide synthase Quaternary Ammonium Compounds 030104 developmental biology Endocrinology medicine.anatomical_structure Oncology chemistry Tumor progression 030220 oncology & carcinogenesis Flavanones biology.protein |
| Zdroj: | Nutrition and cancer. 69(5) |
| ISSN: | 1532-7914 |
| Popis: | Submandibular gland (SMG) is one of the major salivary glands, and is formed by acinar cells that are conveyed to the oral cavity by a duct system. We had previously reported that T2R receptors that were originally identified in gustatory tissues were also present in murine SMG. The addition of bitter compounds to the gland reduced nitric oxide production and downregulated amylase secretion. In this work, we investigated the effect of two different bitter compounds namely denatonium and naringenin on tumor progression as well as the presence of T2R in SCA-9 cells derived from a murine tumor induced in SMG. Both compounds increased tumor cell proliferation in bi- and three-dimensional cultures. These effects were mediated by the activation of arginase and the inhibition of nitric oxide synthase. Denatonium and naringenin also increased vascular endothelial growth factor-A expression via arginase and tumor neovascularization in vivo. T2R6 and T2R4 were identified in SCA-9 cells by immunostaining. Also, Gi and Ggust proteins, which usually couple to T2R receptors, are expressed in these cells. Finally, we demonstrated for the first time that bitter compounds can exert pro-tumor actions that should be taken into account as side effects when they are used as nutraceuticals. |
| Databáze: | OpenAIRE |
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