Molecular analysis of native tissue and whole oils by infrared laser mass spectrometry
| Autor: | Stefan Berkenkamp, Sarah Kruppe, Andreas Rohlfing, Gottfried Pohlentz, Felix Draude, Klaus Dreisewerd |
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| Rok vydání: | 2007 |
| Předmět: |
Spectrophotometry
Infrared Analytical chemistry Carbohydrates Infrared spectroscopy Mass spectrometry Fragaria Sensitivity and Specificity Analytical Chemistry law.invention law Ionization Nanotechnology Sample preparation Phospholipids Triglycerides Flavonoids Chromatography Chemistry Lasers Far-infrared laser Polyatomic ion Laser Freeze Drying Native tissue Seeds Oils |
| Zdroj: | Analytical chemistry. 79(12) |
| ISSN: | 0003-2700 |
| Popis: | We have employed infrared laser desorption ionization orthogonal time-of-flight mass spectrometry (IR-LDI-o-TOF-MS) to generate molecular ion profiles directly from native tissue and from whole oils. The method requires little sample preparation besides an eventual dissection of the areas of interest and drying of particularly water-rich samples. The lateral resolution of the analysis is on the order of the laser focal diameter, and in the third dimension, defined by the depth of material ejection, a few to 10 microm per laser pulse. Various types of small molecules are readily detected from minute volumes of sample. Among these are carbohydrates, phospholipids, triglycerides, and flavonoids. Substantially different molecular profiles were recorded from different areas of a single strawberry seed. |
| Databáze: | OpenAIRE |
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