Developmental capacity of mouse oocytes cryopreserved before and after maturation in vitro
| Autor: | A. K. Champlin, J. J. Eppig, A. C. Schroeder, L. E. Mobraaten |
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| Rok vydání: | 1990 |
| Předmět: |
Ovulation
endocrine system Embryology Cell Survival medicine.medical_treatment media_common.quotation_subject Mice Inbred Strains Fertilization in Vitro Biology Cryopreservation Andrology Mice Endocrinology Human fertilization medicine Animals Ovum media_common Germinal vesicle In vitro fertilisation Obstetrics and Gynecology Embryo Cell Biology Blastomere Oocyte medicine.anatomical_structure Reproductive Medicine Immunology Oocytes Female Follicle Stimulating Hormone |
| Zdroj: | Reproduction. 89:43-50 |
| ISSN: | 1741-7899 1470-1626 |
| Popis: | The survival and developmental capacity of cumulus cell-enclosed oocytes frozen (1) at the germinal vesicle (GV) stage, after maturation in vitro with (2) and without (3) FSH, and (4) after gonadotrophin-stimulated ovulation were assessed. Survival, defined as the number of morphologically normal oocytes, after freeze-thaw at the GV stage (69%), was lower than for oocytes frozen after ovulation (84%), and after maturation in vitro with FSH (88%) and without FSH (81%). Treatment with DMSO without freezing had no effect on survival when compared with untreated controls except in immature GV-stage oocytes for which there was a slight reduction. After insemination in vitro, 9% of frozen-thawed GV-stage oocytes cleaved to two equal blastomeres, but none developed to blastocysts. Of oocytes matured in vitro before freezing, 17% cleaved to the 2-cell stage and 18% of these developed to blastocysts. When oocytes were matured in vitro in the presence of FSH, however, the percentage cleaving to the 2-cell stage after freeze-thaw was improved to 55%, and 77% of 2-cell stage embryos developed to blastocysts. When ovulated cumulus cell-enclosed oocytes were frozen, 88% cleaved and 67% of the cleaved embryos developed to blastocysts. When 158 two-cell embryos resulting from oocytes matured in vitro with FSH were transferred to the oviducts of pseudopregnant foster mothers, 41 genetically marked live young were produced (26%).(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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