In planta effector competition assays detect Hyaloperonospora arabidopsidis effectors that contribute to virulence and localize to different plant subcellular compartments
| Autor: | Sophie J. M. Piquerez, David L. Greenshields, Jonathan D. G. Jones, Ghanasyam Rallapalli, Jorge L. Badel, Georgina Fabro, Naveed Ishaque |
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| Rok vydání: | 2013 |
| Předmět: |
HYALOPERONOSPORA
Physiology Recombinant Fusion Proteins Amino Acid Motifs Arabidopsis Virulence Nicotiana benthamiana Pseudomonas syringae Ciencias Biológicas purl.org/becyt/ford/1 [https] Botany Antibiosis Tobacco purl.org/becyt/ford/1.6 [https] Illumina dye sequencing Plant Diseases Oomycete Hyaloperonospora arabidopsidis biology Effector Electrophoresis Capillary High-Throughput Nucleotide Sequencing Proteins General Medicine Sequence Analysis DNA Bioquímica y Biología Molecular biology.organism_classification ARABIDOPSIS Cell biology Plant Leaves Protein Transport Oomycetes Host-Pathogen Interactions EFFECTORS PSEUDOMONAS Agronomy and Crop Science CIENCIAS NATURALES Y EXACTAS |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 0894-0282 |
| Popis: | The genome of the pathogenic oomycete Hyaloperonospora arabidopsidis is predicted to encode at least 134 high-confidence effectors (HaRxL) carrying the RxLR motif implicated in their translocation into plant cells. However, only four avirulence genes (ATR1, ATR13, ATR5, and ATR39) have been isolated. This indicates that identification of HaRxL effectors based on avirulence is low throughput. We aimed at rapidly identifying H. arabidopsidis effectors that contribute to virulence by developing methods to detect and quantify multiple candidates in bacterial mixed infections using either Illumina sequencing or capillary electrophoresis. In these assays, referred to here as in planta effector competition assays, we estimate the contribution to virulence of individual effectors by calculating the abundance of each HaRxL in the bacterial population recovered from leaves 3 days after inoculation relative to abundance in the initial mixed inoculum. We identified HaRxL that enhance Pseudomonas syringae pv. tomato DC3000 growth in some but not all Arabidopsis accessions. Further analysis showed that HaRxLL464, HaRxL75, HaRxL22, HaRxLL441, and HaRxL89 suppress pathogen-associated molecular pattern-triggered immunity (PTI) and localize to different subcellular compartments in Nicotiana benthamiana, providing evidence for a multilayered suppression of PTI by pathogenic oomycetes and molecular probes for the dissection of PTI. Fil: Badel, Jorge L.. John Innes Institute; Reino Unido Fil: Greenshields, David. John Innes Institute; Reino Unido Fil: Piquerez, Sophie J. M.. John Innes Institute; Reino Unido Fil: Rallapalli, Ganashyam. John Innes Institute; Reino Unido Fil: Fabro, Georgina. John Innes Institute; Reino Unido. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ishaque, Naveed. John Innes Institute; Reino Unido Fil: Jones, Jonathan D. G.. John Innes Institute; Reino Unido |
| Databáze: | OpenAIRE |
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