Bombyx mori nucleopolyhedrovirus ORF101 encodes a budded virus envelope associated protein
| Autor: | Guoping Huang, Keping Chen, Yajing Zhou, Mei Li, Huiqing Chen, Weijun Mai |
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| Rok vydání: | 2014 |
| Předmět: |
viruses
Blotting Western Applied Microbiology and Biotechnology Microbiology Virus Viral envelope Western blot Viral Envelope Proteins medicine Animals Gene biology medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Virus Assembly fungi Proteins General Medicine Bombyx Virology Molecular biology Nucleopolyhedroviruses Blot Molecular Weight Cytosol Polyclonal antibodies Cytoplasm biology.protein |
| Zdroj: | Current microbiology. 69(2) |
| ISSN: | 1432-0991 |
| Popis: | Orf101 (Bm101) of Bombyx mori nucleopolyhedrovirus (BmNPV) is a highly conserved gene in lepidopteran nucleopolyhedroviruses, but its function remains unknown. In this study, Bm101 was characterized. Transcripts of Bm101 were detected from 24 through 96 h post infection (h p.i.) by RT-PCR. The corresponding protein was also detected from 24 to 96 h p.i. in BmNPV-infected BmN cells by Western blot analysis using a polyclonal antibody against Bm101. Western blot assay of occlusion-derived virus and budded virus (BV) preparations revealed that Bm101 encodes a 28-kDa structural protein that is associated with BV and is located in the envelope fraction of budded virions. In addition, confocal analysis showed that the protein was localized in the cytosol and cytoplasmic membrane in virus-infected cells. In conclusion, the available data suggest that Bm101 is a functional ORF of BmNPV and encodes a protein expressed in the late stage of the infection cycle that is associated with the BV envelope. |
| Databáze: | OpenAIRE |
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