In vitro effect of thymosin-alpha1 and interferon-alpha on Th1 and Th2 cytokine synthesis in patients with eAg-negative chronic hepatitis B
| Autor: | E. Grandini, Ranka Vukotic, Elisabetta Loggi, Silvia Galli, Mauro Bernardi, A. Scuteri, Marzia Margotti, Annagiulia Gramenzi, Giovanni Vitale, Carmela Cursaro, Pietro Andreone |
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| Přispěvatelé: | Loggi E, Gramenzi A, Margotti M, Cursaro C, Galli S, Vitale G, Grandini E, Scuteri A, Vukotic R, Andreone P, Bernardi M. |
| Rok vydání: | 2008 |
| Předmět: |
Adult
Male Thymalfasin medicine.medical_treatment Antiviral protein Alpha interferon Hepatitis B Immune response T-helper 1 T-helper 2 Thymosin alpha-1 Antiviral Agents Peripheral blood mononuclear cell Hepatitis B Chronic Th2 Cells Immune system IMMUNE RESPONSE T-HELPER 2 Virology T-HELPER 1 2' 5'-Oligoadenylate Synthetase Humans Medicine Hepatitis B e Antigens Cells Cultured THYMOSIN ALPHA-1 Hepatology business.industry Thymosin Interferon-alpha Middle Aged Th1 Cells medicine.disease In vitro Infectious Diseases Cytokine HEPATITIS B Immunology Leukocytes Mononuclear Interleukin-2 Female Interleukin-4 business |
| Zdroj: | Journal of Viral Hepatitis. 15:442-448 |
| ISSN: | 1365-2893 1352-0504 |
| DOI: | 10.1111/j.1365-2893.2007.00960.x |
| Popis: | Thymosin alpha-1 (Talpha1) has been shown to be effective in chronic hepatitis B treatment. This study investigated the effect of Talpha1 and interferon-alpha (IFNalpha) on cytokine production by peripheral blood mononuclear cells (PBMCs) of 12 patients with eAg-negative chronic hepatitis B (HBV). We evaluated the effect of incubation with Talpha1, IFNalpha or both on the synthesis of T-helper 1 (Th1) cytokines [interleukin-2 (IL-2), IFNgamma] and Th2 cytokines (IL-4, IL-10) and of antiviral protein 2',5'-oligoadenylate synthetase (2',5'-OAS) in patients and in a group of 10 healthy controls. Concerning Th1 profile, controls showed lower IL-2 synthesis than HBV patients. In HBV setting, IFNalpha/Talpha1 combination was able to increase IL-2 production significantly, when compared with baseline condition. About the Th2-cytokines, controls showed statistically lower synthesis of IL-4 and higher production of IL-10, than HBV patients. In these latter, IFNalpha increased the synthesis of IL-10 compared with baseline. Interestingly, both Talpha1 alone and the IFNalpha/Talpha1 combination reversed this effect. Finally, compared with baseline, the synthesis of 2',5'-OAS was significantly higher in the presence of Talpha1 and IFNalpha alone, and in the presence of IFNalpha/Talpha1 association, while no differences were found between controls and HBV patients. In conclusion, in PBMCs from eAg-negative HBV patients, Talpha1 alone was able to increase the antiviral protein synthesis, while in association with IFNalpha, it stimulated the IL-2 synthesis and inhibited the IFN-induced IL-10 production. These results need further investigations, but reinforce the idea of an immunotherapeutic approach for chronic hepatitis B. |
| Databáze: | OpenAIRE |
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