Preparation, Physicochemical Properties, and Transfection Activities of Tartaric Acid-Based Cationic Lipids as Effective Nonviral Gene Delivery Vectors
| Autor: | Yong-Sheng He, Yi-Lin Hou, Xi-Xi Ma, Ning Wan, Chen Li, Si-Yuan Zhou, Yi-Yang Jia, Bang-Le Zhang |
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| Rok vydání: | 2016 |
| Předmět: |
Cell Survival
Green Fluorescent Proteins Pharmaceutical Science 02 engineering and technology Gene delivery 010402 general chemistry 01 natural sciences chemistry.chemical_compound Humans Cationic liposome Tartrates Pharmacology Liposome Gene Transfer Techniques Cationic polymerization DNA General Medicine Transfection 021001 nanoscience & nanotechnology Lipids Combinatorial chemistry 0104 chemical sciences HEK293 Cells chemistry Biochemistry Reagent Aminocaproic Acid Liposomes Tartaric acid lipids (amino acids peptides and proteins) Dodecanol 0210 nano-technology HeLa Cells |
| Zdroj: | Biological & Pharmaceutical Bulletin. 39:1112-1120 |
| ISSN: | 1347-5215 0918-6158 |
| DOI: | 10.1248/bpb.b16-00007 |
| Popis: | In this work two novel cationic lipids using natural tartaric acid as linking backbone were synthesized. These cationic lipids were simply constructed by tartaric acid backbone using head group 6-aminocaproic acid and saturated hydrocarbon chains dodecanol (T-C12-AH) or hexadecanol (T-C16-AH). The physicochemical properties, gel electrophoresis, transfection activities, and cytotoxicity of cationic liposomes were tested. The optimum formulation for T-C12-AH and T-C16-AH was at cationic lipid/dioleoylphosphatidylethanolamine (DOPE) molar ratio of 1 : 0.5 and 1 : 2, respectively, and N/P charge molar ratio of 1 : 1 and 1 : 1, respectively. Under optimized conditions, T-C12-AH and T-C16-AH showed effective gene transfection capabilities, superior or comparable to that of commercially available transfecting reagent 3β-[N-(N',N'-dimethylaminoethyl)carbamoyl]cholesterol (DC-Chol) and N-[2,3-dioleoyloxypropyl]-N,N,N-trimethylammonium chloride (DOTAP). The results demonstrated that the two novel tartaric acid-based cationic lipids exhibited low toxicity and efficient transfection performance, offering an excellent prospect as nonviral vectors for gene delivery. |
| Databáze: | OpenAIRE |
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