WSV181 inhibits JAK/STAT signaling and promotes viral replication in Drosophila
| Autor: | Jianming Chen, Huifang Yuan, Zongliang Huang, Wei Wang, Changkun Pan |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Immunology Virus Replication Virus stat Animals Genetically Modified Immunomodulation Viral Proteins 03 medical and health sciences RNA Virus Infections White spot syndrome virus 1 Downregulation and upregulation Animals Drosophila Proteins Immune Evasion Janus Kinases 030102 biochemistry & molecular biology biology fungi JAK-STAT signaling pathway Viral Load biology.organism_classification DNA Virus Infections Cell biology STAT Transcription Factors 030104 developmental biology Viral replication Dicistroviridae Drosophila Janus kinase Drosophila C virus Viral load Signal Transduction Transcription Factors Developmental Biology |
| Zdroj: | Developmental & Comparative Immunology. 92:20-28 |
| ISSN: | 0145-305X |
| Popis: | The Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway plays a critical role in host defense against viral infections. Here, we report the use of the Drosophila model system to investigate the modulation of the JAK/STAT pathway by the white spot syndrome virus (WSSV) protein WSV181. WSV181 overexpression in transgenic flies resulted in the downregulation of STAT92E and STAT92E-targeted genes. This result indicates that WSV181 can suppress JAK/STAT signaling by controlling STAT92E expression. An infection experiment was carried out on transgenic Drosophila infected with Drosophila C virus and on Litopenaeus vannamei injected with recombinant WSV181 and WSSV. The increased viral load and suppressed transcript levels of JAK/STAT pathway components indicate that WSV181 can promote viral proliferation by inhibiting the JAK/STAT pathway. This study provided evidence for the role of WSV181 in viral replication and revealed a new mechanism through which WSSV evades host immunity to maintain persistent infection. |
| Databáze: | OpenAIRE |
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