Quantification of sunitinib in human plasma by high-performance liquid chromatography–tandem mass spectrometry☆
| Autor: | Ming Zhao, Sharyn D. Baker, Jan Ouwerkerk, Hans Gelderblom, Patton Minkin, Zhaoyuan Chen |
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| Rok vydání: | 2008 |
| Předmět: |
Electrospray
Indoles Chromatography Molecular Structure Calibration curve Chemistry Formic acid Clinical Biochemistry Reproducibility of Results Cell Biology General Medicine Mass spectrometry Tandem mass spectrometry Biochemistry High-performance liquid chromatography Article Analytical Chemistry chemistry.chemical_compound Tandem Mass Spectrometry Sunitinib Humans Pyrroles Sample preparation Quantitative analysis (chemistry) Chromatography High Pressure Liquid |
| Zdroj: | Journal of Chromatography B. 874:84-88 |
| ISSN: | 1570-0232 |
| Popis: | A rapid, sensitive and specific method was developed and validated using LC/MS/MS for determination of sunitinib in human plasma. Sample preparation involved a liquid-liquid extraction by the addition of 0.2 mL of plasma with 4.0 mL tert-butyl-methyl-ether extraction solution containing 25 ng/mL of the internal standard clozapine. Separation of compounds was achieved on a C18 (50 × 2.1 mm i.d., 3.5 µm) analytical column using a mobile phase consisting of acetonitrile/H20 (65:35, v/v) containing 0.1% formic acid and isocratic flow at 0.150 mL/min for 3 minutes. The analytes were monitored by tandem-mass spectrometry with electrospray positive ionization. Linear calibration curves in human plasma were generated over the range of 0.2 to 500 ng/mL with values for the coefficient of determination of >0.9950. Within-and between day precision and accuracy were ≤ 10%. The method was applied to the quantitation of sunitinib in plasma samples from a patient receiving daily oral therapy with sunitinib. |
| Databáze: | OpenAIRE |
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