Expression of matrix metalloproteinase 2 and 9 in breast cancer and breast fibroadenoma: a randomized, double-blind study

Autor: Diego Cipriano Chagas, Francisco Adelton Alves-Ribeiro, Camila Maria Simplício Revoredo, Danylo Rafhael Costa-Silva, Larysse Maira Campos-Verdes, Maria da Conceição Barros-Oliveira, Elmo de Jesus Nery Junior, Carla Solange de Melo Escórcio Dourado, Gilmara Péres Rodrigues, F. Sampaio, Lucia Maria do Rego-Medeiros, Dilina do Nascimento Marreiro, Luana Mota Martins, Luiz Henrique Gebrim, Benedito Borges da Silva
Rok vydání: 2019
Předmět:
Zdroj: Oncotarget
ISSN: 1949-2553
DOI: 10.18632/oncotarget.27347
Popis: // Luana Mota Martins 1 , Carla Solange de Melo Escorcio Dourado 1 , Larysse Maira Campos-Verdes 1 , Fabiane Araujo Sampaio 1 , Camila Maria Simplicio Revoredo 1 , Danylo Rafhael Costa-Silva 1 , Maria da Conceicao Barros-Oliveira 1 , Elmo de Jesus Nery Junior 1 , Lucia Maria do Rego-Medeiros 2 , Luiz Henrique Gebrim 1 , Francisco Adelton Alves-Ribeiro 1 , Gilmara Peres Rodrigues 1 , Diego Cipriano Chagas 1 , Dilina do Nascimento Marreiro 1 and Benedito Borges da Silva 1 , 2 1 Postgraduate Program, Northeast Biotechnology Network (RENORBIO), Biotechnology in Health, Federal University of Piaui, Teresina, Piaui 64000-020, Brazil 2 Facid / Wyden Differential Integral Medicine Faculty, Department of Mastology, Teresina, Piaui 64052-810, Brazill Correspondence to: Benedito Borges da Silva, email: beneditoborges@globo.com Keywords: breast neoplasms; fibroadenoma; matrix metalloproteinase-2; matrix metalloproteinase-9; immunohistochemistry Received: September 17, 2019 Accepted: November 06, 2019 Published: December 03, 2019 ABSTRACT Background Matrix metalloproteinases (MMPs) 2 and 9 may play an important role in cell proliferation and dissemination of cancer. However, few studies have compared the expression of these proteins between breast cancer and fibroadenoma. Material and methods A randomized, double-blind study was carried out in 66 premenopausal women, aged 20-49 years, who had been diagnosed with fibroadenoma or breast cancer. The patients were divided into two groups: Group A, control (fibroadenoma, n=36) and Group B, study (cancer, n=30). Immunohistochemical analysis was performed using tissue samples of fibroadenoma and breast cancer to assess MMP-2 and MMP-9 antigen expression. Cells were considered positive if exhibiting brown cytoplasmic staining. Fisher’s exact test was used to compare the percentage of cases with cells expressing MMP-2 and MMP-9 in control and study groups (p < 0.05). Results Light microscopy showed a higher concentration of cells with positive cytoplasmic staining for MMP-2 and MMP-9 expression in breast cancer than in fibroadenoma. The percentage of cases with cells expressing MMP-2 in the control and study groups was 41.67% and 86.11%, respectively (p < 0.0009), whereas the percentage of cases with cells expressing MMP-9 in groups A and B was 66.67% and 93.33%, respectively (p
Databáze: OpenAIRE