Development and characterization of candidate rotavirus vaccine strains derived from children with diarrhoea in Vietnam
| Autor: | Dang Duc Anh, Phuong M. Nguyen, Baoming Jiang, Luan T. Le, Ngo Thu Huong, Jon R. Gentsch, Nguyen Thi Quynh Huong, Trang Nguyen, Mathew D. Esona, Tran B. Hanh, Paul E. Kilgore, Nguyen Dang Hien, A. Duncan Steele, Yuhuan Wang, Nguyen Thu Huong, Roger I. Glass, Dang N. Ha, Nguyen Van Man |
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| Rok vydání: | 2009 |
| Předmět: |
Diarrhea
Quality Control Rotavirus Virus Cultivation Genes Viral Genotype Vietnamese Developing country Reoviridae medicine.disease_cause Rotavirus Infections Medicine Humans Phylogeny General Veterinary General Immunology and Microbiology biology business.industry Sequence Analysis RNA Public Health Environmental and Occupational Health Rotavirus Vaccines virus diseases Infant biology.organism_classification Virology Rotavirus vaccine language.human_language Rotavirus infection Infectious Diseases Immunization Vietnam language Molecular Medicine RNA Viral medicine.symptom business |
| Zdroj: | Vaccine. 27 |
| ISSN: | 1873-2518 |
| Popis: | In Vietnam, rotavirus infection accounts for more than one-half of all hospitalizations for diarrhoea among children less than 5 years of age. While new vaccines to prevent rotavirus diarrhoea have been developed and introduced into some countries by multinational manufacturers, the ability for developing countries such as Vietnam to introduce several new and important vaccines into the routine infant immunization schedule may be challenging. In order to be partially self-sufficient in vaccine production, Vietnam has pursued the development of several rotavirus strains as candidate vaccines using isolates obtained from Vietnamese children with diarrhoea. This paper describes the origin, isolation and characterization of 3 human rotavirus strains being considered for further vaccine development in Vietnam. The goal is to prepare a monovalent G1P [8] rotavirus vaccine using one of these strains obtained in Vietnam and naturally attenuated by multiple passages in cell culture. While this is an ambitious project that will require several years' work, we are using the lessons learned to improve the overall quality of vaccine production including the use of Vero cell techniques for the manufacture of other vaccines in Vietnam. |
| Databáze: | OpenAIRE |
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