Culture-Independent Analysis of Indomethacin-Induced Alterations in the Rat Gastrointestinal Microbiota
| Autor: | Alison M. Bendele, Allison L. St. Amand, Norman R. Pace, Andrew B. Dalby, Daniel N. Frank |
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| Rok vydání: | 2006 |
| Předmět: |
Peptic Ulcer
Gastrointestinal Diseases Indomethacin Molecular Sequence Data Clone (cell biology) Biology Pharmacology Applied Microbiology and Biotechnology Enterococcus faecalis Microbial Ecology Intestine Small medicine Animals Mesenteric lymph nodes Adverse effect Ecosystem Ecology Gastrointestinal microbiota Anti-Inflammatory Agents Non-Steroidal biology.organism_classification digestive system diseases Small intestine Rats medicine.anatomical_structure Real-time polymerase chain reaction Rats Inbred Lew Immunology Female Culture independent Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 72:6707-6715 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.00378-06 |
| Popis: | Nonsteroidal anti-inflammatory drugs (NSAIDs) are commonly prescribed for a variety of inflammatory conditions; however, the benefits of this class of drugs are accompanied by deleterious side effects, most commonly gastric irritation and ulceration. NSAID-induced ulceration is thought to be exacerbated by intestinal microbiota, but previous studies have not identified specific microbes that contribute to these adverse effects. In this study, we conducted a culture-independent analysis of ∼1,400 bacterial small-subunit rRNA genes associated with the small intestines and mesenteric lymph nodes of rats treated with the NSAID indomethacin. This is the first molecular analysis of the microbiota of the rat small intestine. A comparison of clone libraries and species-specific quantitative PCR results from rats treated with indomethacin and untreated rats revealed that organisms closely related to Enterococcus faecalis were heavily enriched in the small intestine and mesenteric lymph nodes of the treated rats. These data suggest that treatment of NSAID-induced ulceration may be facilitated by addressing the microbiological imbalances. |
| Databáze: | OpenAIRE |
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