Evaluation of Haptoglobin and Its Proteoforms as Glioblastoma Markers

Autor: Fedor Kabachenko, Olga K. Legina, Stanislav N. Naryzhny, Rimma Pantina, Elena Zorina, Natalia L. Ronzhina, Nikolai Klopov, Viktor Zgoda, Maria G. Zavialova
Rok vydání: 2021
Předmět:
0301 basic medicine
Proteomics
QH301-705.5
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
0302 clinical medicine
Tandem Mass Spectrometry
zonulin
Cell Line
Tumor
Blood plasma
Biomarkers
Tumor
Humans
Biology (General)
Physical and Theoretical Chemistry
QD1-999
Molecular Biology
Spectroscopy
chemistry.chemical_classification
Two-dimensional gel electrophoresis
biology
Haptoglobins
Organic Chemistry
Haptoglobin
Zonulin
Computational Biology
General Medicine
Prognosis
Phenotype
haptoglobin
Computer Science Applications
Blot
Gene Expression Regulation
Neoplastic
Chemistry
030104 developmental biology
chemistry
030220 oncology & carcinogenesis
Proteolysis
biology.protein
Cancer research
Biomarker (medicine)
biomarker
Disease Susceptibility
Glycoprotein
Glioblastoma
Chromatography
Liquid
Zdroj: International Journal of Molecular Sciences
Volume 22
Issue 12
International Journal of Molecular Sciences, Vol 22, Iss 6533, p 6533 (2021)
ISSN: 1422-0067
Popis: Haptoglobin (Hp) is a blood plasma glycoprotein that plays a critical role in tissue protection and the prevention of oxidative damage. Haptoglobin is an acute-phase protein, its concentration in plasma changes in pathology, and the test for its concentration is part of normal clinical practice. Haptoglobin is a conservative protein and is the subject of research as a potential biomarker of many diseases, including malignant neoplasms. The Human Hp gene is polymorphic and controls the synthesis of three major phenotypes—homozygous Hp1-1 and Hp2-2, and heterozygous Hp2-1, determined by a combination of allelic variants that are inherited. Numerous studies indicate that the phenotype of haptoglobin can be used to judge the individual’s predisposition to various diseases. In addition, Hp undergoes various post-translational modifications (PTMs). Glioblastoma multiform (GBM) is the most malignant primary brain tumor. In our study, we have analyzed the state of Hp proteoforms in plasma and cells using 1D (SDS-PAGE) and 2D electrophoresis (2DE) with the following mass spectrometry (LC ES-MS/MS) or Western blotting. We found that the levels of α2- and β-chain proteoforms are up-regulated in the plasma of GBM patients. An unprocessed form of Hp2-2 (PreHp2-2, zonulin) with unusual biophysical parameters (pI/Mw) was also detected in the plasma of GBM patients and glioblastoma cells. Altogether, this data shows the possibility to use proteoforms of haptoglobin as a potential GBM-specific plasma biomarker.
Databáze: OpenAIRE
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