Insulin-responsive Nuclear Proteins Facilitate Sp1 Interactions with the Insulin-like Growth Factor-I Gene
Autor: | Ching-I Pao, Elizabeth N. Kaytor, Lawrence S. Phillips, Juan Li Zhu |
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Rok vydání: | 2001 |
Předmět: |
Male
Sp1 Transcription Factor medicine.medical_treatment Biology Biochemistry Rats Sprague-Dawley Transactivation Insulin-like growth factor Transcription (biology) In vivo Consensus Sequence medicine Animals Insulin Insulin-Like Growth Factor I Nuclear protein Molecular Biology Cell Nucleus Binding Sites Binding protein Growth factor Nuclear Proteins DNA Cell Biology AT Rich Sequence Molecular biology GC Rich Sequence Rats Hepatocytes |
Zdroj: | Journal of Biological Chemistry. 276:36896-36901 |
ISSN: | 0021-9258 |
DOI: | 10.1074/jbc.m104035200 |
Popis: | The diabetes-induced decrease in insulin-like growth factor-I transcription appears to be mediated by footprint region V in exon 1. Since region V contains both an Sp1 site and an AT-rich element that recognizes an insulin-responsive binding protein (IRBP), we tested the hypothesis that Sp1 interactions are facilitated by an IRBP. Binding of nuclear extracts to region V probes was reduced by mutational or chemical interference with the AT-rich element. Blocking the AT site also reduced interactions of Sp1 with region V in vitro and blunted transactivation of region V reporter constructs by Sp1 in vivo. Sp1 binding was enhanced by small quantities of hepatic nuclear extracts, but enhancement was reduced by the AT mutation and abolished by a 5-base pair insertion between the AT-rich and GC-rich sites, and transactivation by Sp1 in vivo was diminished by inserting bases between the AT-rich and GC-rich elements. However, treating cells with insulin increased the ability of nuclear extracts to enhance Sp1 binding. These findings indicate that the presence of the AT-rich element is essential for the actions of Sp1 in vitro and in vivo, and the combination of both spacing requirements and insulin responsiveness suggests that IRBP may interact directly with Sp1. |
Databáze: | OpenAIRE |
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