Sympathetic innervation controls homeostasis of neuromuscular junctions in health and disease

Autor: Till Marquardt, Rüdiger Rudolf, Yasmin Issop, Dan Cox, Emily O'Connor, Isis do Carmo Kettelhut, Luiz Carlos Carvalho Navegantes, Veit Witzemann, Tullio Pozzan, Jordi Molgó, Dittmar Labeit, Muzamil Majid Khan, Evelyne Benoit, Franziska Wild, Danilo Lustrino, Hanns Lochmüller, Markus Reischl, Willian A. Silveira, Siegfried Labeit, Tatjana Straka
Přispěvatelé: Institut des Neurosciences Paris-Saclay (NeuroPSI), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Service d'Ingénierie Moléculaire pour la Santé (ex SIMOPRO) (SIMoS), Médicaments et Technologies pour la Santé (MTS), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Male
Sympathetic nervous system
Sympathetic Nervous System
medicine.medical_treatment
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Neuromuscular junction
Biosensing Techniques
Corrections
Synapse
0302 clinical medicine
Postsynaptic potential
Cyclic AMP
Medicine
Homeostasis
Sympathetic neurons
Neurons
Multidisciplinary
[SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior
[SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences
Biological Sciences
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
medicine.anatomical_structure
Phenotype
Health
Myasthenia
Female
SISTEMA NERVOSO SIMPÁTICO
Signal Transduction
Beta-agonists
CAMP
Active Transport
Cell Nucleus
Models
Biological
03 medical and health sciences
Animals
Sympathectomy
Muscle
Skeletal
Cell Nucleus
business.industry
Skeletal muscle
Mice
Inbred C57BL
Electrophysiology
030104 developmental biology
Nervous System Diseases
business
Neuroscience
030217 neurology & neurosurgery
Transcription Factors
Zdroj: Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2016, 113 (3), pp.746-50. ⟨10.1073/pnas.1524272113⟩
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Proceedings of the National Academy of Sciences of the United States of America, 2016, 113 (3), pp.746-50. ⟨10.1073/pnas.1524272113⟩
ISSN: 0027-8424
1091-6490
Popis: International audience; The distribution and function of sympathetic innervation in skeletal muscle have largely remained elusive. Here we demonstrate that sympathetic neurons make close contact with neuromuscular junctions and form a network in skeletal muscle that may functionally couple different targets including blood vessels, motor neurons, and muscle fibers. Direct stimulation of sympathetic neurons led to activation of muscle postsynaptic β2-adrenoreceptor (ADRB2), cAMP production, and import of the transcriptional coactivator peroxisome proliferator-activated receptor γ-coactivator 1α (PPARGC1A) into myonuclei. Electrophysiological and morphological deficits of neuromuscular junctions upon sympathectomy and in myasthenic mice were rescued by sympathicomimetic treatment. In conclusion, this study identifies the neuromuscular junction as a target of the sympathetic nervous system and shows that sympathetic input is crucial for synapse maintenance and function.
Databáze: OpenAIRE
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