Establishment of 11 linked X-STR loci within 1.1 Mb to assist with kinship testing
| Autor: | Yu-Jen Yu, Keng-Hsien Liao, Chun-Yen Lin, James Chun-I Lee, Adrian Linacre, Li-Chin Tsai, Hsing-Mei Hsieh |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Forensic Genetics Male Linkage disequilibrium Locus (genetics) Biology Polymerase Chain Reaction DNA sequencing Linkage Disequilibrium Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Gene Frequency Humans 030216 legal & forensic medicine Allele X chromosome Genetics Chromosomes Human X Polymorphism Genetic Haplotype Sequence Analysis DNA 030104 developmental biology Genetic Loci GenBank Microsatellite Female Microsatellite Repeats |
| Zdroj: | International journal of legal medicine. 132(4) |
| ISSN: | 1437-1596 |
| Popis: | This report identifies and characterizes 10 novel short tandem repeat (STR) loci on the human X chromosome, all of which are within a range of 1.1 Mb. These newly characterized loci were developed to aid in kinship assignment when the X chromosome is specifically required. The repeat DNA sequences were identified initially using data in GenBank and are located immediately upstream and downstream from the previously described locus DXS6807. Only those loci with seven or more observed alleles were used for further study resulting in the identification of 10 new loci. The distance between each pair of loci ranged from 24,998 to 244,701 bp with an average of approximately 110.8 kb. The number of observed alleles ranged from 7 to 30 for these 10 loci with a polymorphic information content ranging from 0.593 to 0.930. The LOD score from a pairwise linkage study ranged from 4.40 to 23.73, indicating that these 11 loci were highly linked, as expected. In line with standard forensic practice, all 11 loci can be amplified in one multiplex reaction, and comprehensive allelic ladders for all the loci have been constructed. These newly established 11 linked STR loci on the human X chromosome were found to be highly polymorphic and have the potential to aid in kinship testing where the X chromosome loci currently plays a role. |
| Databáze: | OpenAIRE |
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