Enzymes of anaerobic ethylbenzene and p-ethylphenol catabolism in 'Aromatoleum aromaticum': differentiation and differential induction
| Autor: | Lina Clermont, Katharina Sünwoldt, Georg Schmitt, Daniel Kleinsorge, Enrico Muhr, Johann Heider, Karola Schühle, Iris Schall, Paul R. F. Cordero, Jörg Kahnt, Deniz Seyhan |
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| Rok vydání: | 2015 |
| Předmět: |
Rhodocyclaceae
Biochemistry Microbiology Ethylbenzene Mixed Function Oxygenases Hydroxylation chemistry.chemical_compound Phenols Genetics Benzene Derivatives Anaerobiosis Enzyme inducer Molecular Biology Heme Alcohol dehydrogenase chemistry.chemical_classification biology Catabolism General Medicine Metabolic pathway Enzyme chemistry Enzyme Induction biology.protein Metabolic Networks and Pathways |
| Zdroj: | Archives of microbiology. 197(9) |
| ISSN: | 1432-072X |
| Popis: | The denitrifying bacterium 'Aromatoleum aromaticum' strain EbN1 is one of the best characterized bacteria regarding anaerobic ethylbenzene degradation. EbN1 also degrades various other aromatic and phenolic compounds in the absence of oxygen, one of them being p-ethylphenol. Despite having similar chemical structures, ethylbenzene and p-ethylphenol have been proposed to be metabolized by completely separate pathways. In this study, we established and applied biochemical and molecular biological methods to show the (almost) exclusive presence and specificity of enzymes involved in the respective degradation pathways by recording enzyme activities, complemented by heme staining, immuno- and biotin-blotting analyses. These combined results substantiated the predicted p-ethylphenol degradation pathway. The identified enzymes include a heme c-containing p-ethylphenol-hydroxylase, both an (R)- and an (S)-specific alcohol dehydrogenase as well as a novel biotin-dependent carboxylase. We also establish an activity assay for benzoylacetate-CoA ligases likely being involved in both metabolic pathways. |
| Databáze: | OpenAIRE |
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